RNA Editing of Sorghum Mitochondrial Atp6 Transcripts Changes 15 Amino Acids and Generates a Carboxy-terminus Identical to Yeast

Overview

Journal Curr Genet

Specialty Genetics

Date 1991 Nov 1

PMID 1839673

Citations 31

Authors

F Kempken

J A Mullen

D R Pring

H. V. Tang

Affiliations

Soon will be listed here.

Abstract

Sequencing of sorghum mitochondrial atp6 cDNA clones revealed 19 C-to-U transcript editing events within a 756 bp-conserved core gene; three were silent and 16 resulted in 15 amino acid changes. Only one edit, which was silent, was found in the 381 bp amino-extension to the core gene. Eleven of the 15 changed amino acids were identical with or else represented conservative changes compared to yeast atp6. Editing of a CAA codon to TAA truncates the carboxy-terminus to a position identical to that of yeast. The frequency of editing at sites which change amino acids was very high in contrast to partial editing at silent, third base, sites.

Citing Articles

A Systematic Review and Developmental Perspective on Origin of CMS Genes in Crops.

Zhang X, Ding Z, Lou H, Han R, Ma C, Yang S Int J Mol Sci. 2024; 25(15).

PMID: 39125940 PMC: 11312923. DOI: 10.3390/ijms25158372.

RNA editing: only eleven sites are present in the Physcomitrella patens mitochondrial transcriptome and a universal nomenclature proposal.

Rudinger M, Funk H, Rensing S, Maier U, Knoop V Mol Genet Genomics. 2009; 281(5):473-81.

PMID: 19169711 DOI: 10.1007/s00438-009-0424-z.

Did RNA editing in plant organellar genomes originate under natural selection or through genetic drift?.

Jobson R, Qiu Y Biol Direct. 2008; 3:43.

PMID: 18939975 PMC: 2584032. DOI: 10.1186/1745-6150-3-43.

Patterns of partial RNA editing in mitochondrial genes of Beta vulgaris.

Mower J, Palmer J Mol Genet Genomics. 2006; 276(3):285-93.

PMID: 16862402 DOI: 10.1007/s00438-006-0139-3.

The organization of mitochondrial atp6 gene region in male fertile and CMS lines of pepper (Capsicum annuum L.).

Kim D, Kim B Curr Genet. 2005; 49(1):59-67.

PMID: 16328502 DOI: 10.1007/s00294-005-0032-3.

References

Covello P, Gray M . RNA editing in plant mitochondria. Nature. 1989; 341(6243):662-6. DOI: 10.1038/341662a0. View

Schuster W, Unseld M, Wissinger B, Brennicke A . Ribosomal protein S14 transcripts are edited in Oenothera mitochondria. Nucleic Acids Res. 1990; 18(2):229-33. PMC: 330258. DOI: 10.1093/nar/18.2.229. View

Bland M, Levings 3rd C, Matzinger D . The ATPase subunit 6 gene of tobacco mitochondria contains an unusual sequence. Curr Genet. 1987; 12(7):475-81. DOI: 10.1007/BF00419555. View

Gualberto J, Weil J, Grienenberger J . Editing of the wheat coxIII transcript: evidence for twelve C to U and one U to C conversions and for sequence similarities around editing sites. Nucleic Acids Res. 1990; 18(13):3771-6. PMC: 331076. DOI: 10.1093/nar/18.13.3771. View

Makaroff C, Apel I, Palmer J . The atp6 coding region has been disrupted and a novel reading frame generated in the mitochondrial genome of cytoplasmic male-sterile radish. J Biol Chem. 1989; 264(20):11706-13. View

Macfarlane J, Wahleithner J, WOLSTENHOLME D . A broad bean mitochondrial atp6 gene with an unusually simple, non-conserved 5' region. Curr Genet. 1990; 18(1):87-91. DOI: 10.1007/BF00321121. View

Grabau E, Havlik M, Gesteland R . Chimeric organization of two genes for the soybean mitochondrial ATPase subunit 6. Curr Genet. 1988; 13(1):83-9. DOI: 10.1007/BF00365761. View

Hiesel R, Wissinger B, Brennicke A . Cytochrome oxidase subunit II mRNAs in Oenothera mitochondria are edited at 24 sites. Curr Genet. 1990; 18(4):371-5. DOI: 10.1007/BF00318219. View

Chen S, Habib G, Yang C, Gu Z, Lee B, Weng S . Apolipoprotein B-48 is the product of a messenger RNA with an organ-specific in-frame stop codon. Science. 1987; 238(4825):363-6. DOI: 10.1126/science.3659919. View

10.

Hiesel R, Wissinger B, Schuster W, Brennicke A . RNA editing in plant mitochondria. Science. 1989; 246(4937):1632-4. DOI: 10.1126/science.2480644. View

11.

Macino G, Tzagoloff A . Assembly of the mitochondrial membrane system: sequence analysis of a yeast mitochondrial ATPase gene containing the oli-2 and oli-4 loci. Cell. 1980; 20(2):507-17. DOI: 10.1016/0092-8674(80)90637-6. View

12.

Powell L, Wallis S, Pease R, Edwards Y, Knott T, Scott J . A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine. Cell. 1987; 50(6):831-40. DOI: 10.1016/0092-8674(87)90510-1. View

13.

Graves P, Begu D, Velours J, Neau E, Belloc F, Litvak S . Direct protein sequencing of wheat mitochondrial ATP synthase subunit 9 confirms RNA editing in plants. J Mol Biol. 1990; 214(1):1-6. DOI: 10.1016/0022-2836(90)90138-c. View

14.

Dewey R, Levings C, Timothy D . Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol. 1985; 79(3):914-9. PMC: 1074995. DOI: 10.1104/pp.79.3.914. View

15.

Feagin J, Abraham J, Stuart K . Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma brucei. Cell. 1988; 53(3):413-22. DOI: 10.1016/0092-8674(88)90161-4. View

16.

Begu D, Graves P, Domec C, Arselin G, Litvak S, Araya A . RNA editing of wheat mitochondrial ATP synthase subunit 9: direct protein and cDNA sequencing. Plant Cell. 1990; 2(12):1283-90. PMC: 159973. DOI: 10.1105/tpc.2.12.1283. View

17.

Walker J, Saraste M, Gay N . The unc operon. Nucleotide sequence, regulation and structure of ATP-synthase. Biochim Biophys Acta. 1984; 768(2):164-200. DOI: 10.1016/0304-4173(84)90003-x. View

18.

Gualberto J, Lamattina L, Bonnard G, Weil J, Grienenberger J . RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature. 1989; 341(6243):660-2. DOI: 10.1038/341660a0. View

19.

Weissmann C, Cattaneo R, Billeter M . RNA editing. Sometimes an editor makes sense. Nature. 1990; 343(6260):697-9. DOI: 10.1038/343697a0. View

20.

Saiki R, Gelfand D, Stoffel S, Scharf S, Higuchi R, Horn G . Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988; 239(4839):487-91. DOI: 10.1126/science.2448875. View