Direct Proteasome-independent Cross-presentation of Viral Antigen by Plasmacytoid Dendritic Cells on Major Histocompatibility Complex Class I

Overview

Journal Nat Immunol

Date 2008 Apr 1

PMID 18376401

Citations 141

Authors

Tiziana Di Pucchio

Bithi Chatterjee

Anna Smed-Sorensen

Sandra Clayton

Adam Palazzo

Monica Montes

Yaming Xue

Ira Mellman

Jacques Banchereau

John E Connolly

Affiliations

Soon will be listed here.

Abstract

Although plasmacytoid dendritic cells (pDCs) respond to virus replication in a nonspecific way by producing large amounts of type I interferon, a rapid, direct function for pDCs in activating antiviral lymphocytes is less apparent. Here we show that pDCs were able to rapidly initiate antigen-specific antiviral CD8+ T cell responses. After being exposed to virus, pDCs efficiently and rapidly internalized exogenous viral antigens and then presented those antigens on major histocompatibility complex (MHC) class I to CD8+ T cells. Processing of exogenous antigen occurred in endocytic organelles and did not require transit of antigen to the cytosol. Intracellular stores of MHC class I partially localized together with the transferrin receptor and internalized transferrin in endosomes, which suggested that such recycling endosomes are sites for loading peptide onto MHC class I or for peptide transit. Our data demonstrate that pDCs use 'ready-made' stores of MHC class I to rapidly present exogenous antigen to CD8+ T cells.

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