Novel Fold and Putative Receptor Binding Site of Granulocyte-macrophage Colony-stimulating Factor

Overview

Journal Science

Specialty Science

Date 1991 Dec 20

PMID 1837174

Citations 40

Authors

K Diederichs

T Boone

P A Karplus

Affiliations

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Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates the development of and the cytotoxic activity of white blood cells. Recombinant human GM-CSF has proven useful in the treatment of blood disorders. The structure of GM-CSF, which was determined at 2.4 angstrom resolution by x-ray crystallography, has a novel fold combining a two-stranded antiparallel beta sheet with an open bundle of four alpha helices. Residues implicated in receptor recognition, which are distant in the primary sequence, are on adjacent alpha helices in the folded protein. A working model for the receptor binding site is presented.

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