Hematopoietic Fingerprints: an Expression Database of Stem Cells and Their Progeny

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2008 Mar 29

PMID 18371395

Citations 207

Authors

Stuart M Chambers

Nathan C Boles

Kuan-Yin K Lin

Megan P Tierney

Teresa V Bowman

Steven B Bradfute

Alice J Chen

Akil A Merchant

Olga Sirin

David C Weksberg

Mehveen G Merchant

C Joseph Fisk

Chad A Shaw

Margaret A Goodell

Affiliations

Soon will be listed here.

Abstract

Hematopoietic stem cells (HSCs) continuously regenerate the hematologic system, yet few genes regulating this process have been defined. To identify candidate factors involved in differentiation and self-renewal, we have generated an expression database of hematopoietic stem cells and their differentiated progeny, including erythrocytes, granulocytes, monocytes, NK cells, activated and naive T cells, and B cells. Bioinformatic analysis revealed HSCs were more transcriptionally active than their progeny and shared a common activation mechanism with T cells. Each cell type also displayed unique biases in the regulation of particular genetic pathways, with Wnt signaling particularly enhanced in HSCs. We identified approximately 100-400 genes uniquely expressed in each cell type, termed lineage "fingerprints." In overexpression studies, two of these genes, Zfp 105 from the NK cell lineage, and Ets2 from the monocyte lineage, were able to significantly influence differentiation toward their respective lineages, demonstrating the utility of the fingerprints for identifying genes that regulate differentiation.

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