Mesna Protects Splanchnic Organs from Oxidative Stress Induced by Pneumoperitoneum

Overview

Journal Surg Endosc

Publisher Springer

Specialties Gastroenterology
General Surgery
Radiology

Date 2008 Mar 28

PMID 18368452

Citations 5

Authors

Petros Ypsilantis

Ioannis Tentes

Konstantinos Anagnostopoulos

Alexandros Kortsaris

Constantinos Simopoulos

Affiliations

Soon will be listed here.

Abstract

Background: We investigated the potential beneficial effect of the antioxidant 2-mercaptoethane-sulfonate (mesna) against oxidative stress induced by pneumoperitoneum in splanchnic organs.

Methods: Wistar rats were subjected to either (a) CO(2) pneumoperitoneum (15 mmHg for 60 min) (group P), (b) pretreatment with mesna (400 mg/kg, p.o.) followed by pneumoperitoneum with a 180 min interval (group MP), (c) sham operation (group S), or (d) administration of mesna only (group M). Forty-five minutes after desufflation (groups P and MP), 60 + 45 min after the induction of anesthesia (group S), or 180 min after mesna administration (group M), tissue specimens were excised from liver, kidneys, jejunum and stomach. Tissue oxidative state was assessed on the basis of glutathione-to-glutathione disulfide ratio, malondialdehyde concentration , and superoxide dismutase activity.

Results: Pneumoperitoneum deteriorated all the oxidative stress markers in the organs studied. Mesna prevented the occurrence of oxidative stress following pneumoperitoneum in all the organs studied. In the absence of pneumoperitoneum, the administration of mesna caused mild enhancement of the oxidative state of liver, stomach, and kidneys compared to sham controls.

Conclusions: Prophylaxis with mesna prevents oxidative stress induced by pneumoperitoneum in splanchnic organs.

Citing Articles

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Shi J, Du S, Yu J, Zhang Y, He S, Dong S Oxid Med Cell Longev. 2021; 2021:9034376.

PMID: 33927798 PMC: 8053056. DOI: 10.1155/2021/9034376.

Room air versus carbon dioxide pneumoperitoneum: effects on oxidative state, apoptosis and histology of splanchnic organs.

Ypsilantis P, Lambropoulou M, Tentes I, Chryssidou M, Georgantas T, Simopoulos C Surg Endosc. 2015; 30(4):1388-95.

PMID: 26123338 DOI: 10.1007/s00464-015-4341-4.

Prevention of pulmonary complications of pneumoperitoneum in rats.

Karapolat S, Gezer S, Yildirim U, Dumlu T, Karapolat B, Ozaydin I J Cardiothorac Surg. 2011; 6:14.

PMID: 21303502 PMC: 3044100. DOI: 10.1186/1749-8090-6-14.

Carbon dioxide pneumoperitoneum induces anti-inflammatory response and hepatic oxidative stress in young rats with bacterial peritonitis.

Hsieh C, Tain Y, Chen Y, Chang K, Jean Y, Huang L Pediatr Surg Int. 2010; 27(3):289-94.

PMID: 21110033 DOI: 10.1007/s00383-010-2801-4.

The impact of carbon dioxide pneumoperitoneum on liver regeneration after liver resection in a rat model.

Schmidt S, Schumacher G, Klage N, Chopra S, Neuhaus P, Neumann U Surg Endosc. 2009; 24(1):1-8.

PMID: 19533243 DOI: 10.1007/s00464-009-0536-x.

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