Purification and Characterization of Equine Infectious Anemia Virus

Overview

Journal Arch Virol

Specialty Microbiology

Date 1976 Jan 1

PMID 183628

Citations 7

Authors

H D MATHEKA

L COGGINS

J N SHIVELY

N L Norcross

Affiliations

Soon will be listed here.

Abstract

EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.

Citing Articles

Equine infectious anemia.

Sellon D Vet Clin North Am Equine Pract. 1993; 9(2):321-36.

PMID: 8395326 PMC: 7126748. DOI: 10.1016/s0749-0739(17)30399-1.

The immunopathogenesis of equine infectious anemia virus.

Sellon D, Fuller F, McGuire T Virus Res. 1994; 32(2):111-38.

PMID: 8067050 PMC: 7134051. DOI: 10.1016/0168-1702(94)90038-8.

Structural proteins of equine infectious anemia virus.

Cheevers W, Ackley C, Crawford T J Virol. 1978; 28(3):997-1001.

PMID: 215790 PMC: 525823. DOI: 10.1128/JVI.28.3.997-1001.1978.

Detection of proviral DNA in horse cells infected with equine infectious anemia virus.

Rice N, Simek S, Ryder O, COGGINS L J Virol. 1978; 26(3):577-83.

PMID: 209211 PMC: 525883. DOI: 10.1128/JVI.26.3.577-583.1978.

Electron microscopic studies on equine infectious anemia virus (EIAV). Brief report.

Weiland F, MATHEKA H, COGGINS L, Hatner D Arch Virol. 1977; 55(4):335-40.

PMID: 202230 DOI: 10.1007/BF01315055.

References

Nakajima H, Norcross N, COGGINS L . Demonstration of antigenic identity between purified equine infectious anemia virus and an antigen extracted from infected horse spleen. Infect Immun. 1972; 6(3):416-7. PMC: 422550. DOI: 10.1128/iai.6.3.416-417.1972. View

Kono Y, Yoshino T . Propagation of equine infectious anemia virus in horse kidney cell cultures. Natl Inst Anim Health Q (Tokyo). 1974; 14(4):155-62. View

Tajima M, Nakajima H, Ito Y . Electron microscopy of equine infectious anemia virus. J Virol. 1969; 4(4):521-7. PMC: 375902. DOI: 10.1128/JVI.4.4.521-527.1969. View

COGGINS L, Norcross N . Immunodiffusion reaction in equine infectious anemia. Cornell Vet. 1970; 60(2):330-5. View

Nakajima H, Tajima M, Tanaka S, Ushimi C . Physicochemical studies of equine infectionus anemia virus. 3. Purification and electron microscopic observation of the virus. Arch Gesamte Virusforsch. 1969; 28(3):348-60. DOI: 10.1007/BF01240949. View

MALMQUIST W, Barnett D, Becvar C . Production of equine infectious anemia antigen in a persistently infected cell line. Arch Gesamte Virusforsch. 1973; 42(4):361-70. DOI: 10.1007/BF01250717. View

Ito Y, Kono Y, Kobayashi K . Electron microscopic observations of equine infectious anemia (EIA) virus in cultivated horse leukocytes. (Brief report). Arch Gesamte Virusforsch. 1969; 28(3):411-6. DOI: 10.1007/BF01240954. View

Nakajima H, Yoshino T, Ushimi C . Equine infectious anemia virus from infected horse serum. Infect Immun. 1974; 10(3):667-8. PMC: 423001. DOI: 10.1128/iai.10.3.667-668.1974. View

Kobayashi K, Kono Y . Propagation and titration of equine infectious anemia virus in horse leukocyte culture. Natl Inst Anim Health Q (Tokyo). 1967; 7(1):8-20. View

10.

Nakajima H, Ushimi C . Immunodiffusion studies of purified equine infectious anemia virus. Infect Immun. 1971; 3(3):373-7. PMC: 416160. DOI: 10.1128/iai.3.3.373-377.1971. View

11.

McEwen C . Tables for estimating sedimentation through linear concentration gradients of sucrose solution. Anal Biochem. 1967; 20(1):114-49. DOI: 10.1016/0003-2697(67)90271-0. View

12.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

13.

Crawford T, McGuire T, Henson J . Detection of equine infectious anemia virus in vitro by immunofluorescence. Arch Gesamte Virusforsch. 1971; 34(4):332-9. DOI: 10.1007/BF01242979. View