Recovery, Purification, and Cloning of High-molecular-weight DNA from Soil Microorganisms

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2008 Mar 25

PMID 18359830

Citations 24

Authors

Mark R Liles

Lynn L Williamson

Jitsupang Rodbumrer

Vigdis Torsvik

Robert M Goodman

Jo Handelsman

Affiliations

Soon will be listed here.

Abstract

We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries.

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