Ultrasound-guided in Utero Injections Allow Studies of the Development and Function of the Eye

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2008 Mar 21

PMID 18351670

Citations 17

Authors

Claudio Punzo

Constance L Cepko

Affiliations

Soon will be listed here.

Abstract

Ultrasound-guided in utero injections into the brain of murine embryos has been shown to facilitate gene delivery. We investigated whether these methods would allow gene transfer into ocular structures. Gene transfer using retroviral vectors or electroporation was found to be quite effective. We determined the window of time, as well as compared several strains of mice, that yield a high degree of survival and successful gene transfer. Several retroviral constructs were tested for expression and coexpresssion of two genes in retinal cell types. In addition, a retroviral vector was engineered to give cone photoreceptor-enriched expression, and a retroviral vector was demonstrated to provide RNAi-mediated loss-of-function. These methods enable access to early ocular structures and provide a more rapid method of assessment of gene and promoter function than possible using genetically engineered mice.

Citing Articles

Rod Outer Segment Development Influences AAV-Mediated Photoreceptor Transduction After Subretinal Injection.

Petit L, Ma S, Cheng S, Gao G, Punzo C Hum Gene Ther. 2017; 28(6):464-481.

PMID: 28510482 PMC: 5488363. DOI: 10.1089/hum.2017.020.

Optical coherence tomography guided microinjections in live mouse embryos: high-resolution targeted manipulation for mouse embryonic research.

Syed S, Coughlin A, Garcia M, Wang S, West J, Larin K J Biomed Opt. 2015; 20(5):051020.

PMID: 25581495 PMC: 4405081. DOI: 10.1117/1.JBO.20.5.051020.

A relative shift in cloacal location repositions external genitalia in amniote evolution.

Tschopp P, Sherratt E, Sanger T, Groner A, Aspiras A, Hu J Nature. 2014; 516(7531):391-4.

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Lineage analysis of the late otocyst stage mouse inner ear by transuterine microinjection of a retroviral vector encoding alkaline phosphatase and an oligonucleotide library.

Jiang H, Wang L, Beier K, Cepko C, Fekete D, Brigande J PLoS One. 2013; 8(7):e69314.

PMID: 23935981 PMC: 3723842. DOI: 10.1371/journal.pone.0069314.

Retinal gene delivery by rAAV and DNA electroporation.

Venkatesh A, Ma S, Langellotto F, Gao G, Punzo C Curr Protoc Microbiol. 2013; Chapter 14:Unit 14D.4.

PMID: 23408132 PMC: 3643812. DOI: 10.1002/9780471729259.mc14d04s28.

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