Structural and Functional Analysis of the HSP90AA1 Gene: Distribution of Polymorphisms Among Sheep with Different Responses to Scrapie

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2008 Mar 19

PMID 18347938

Citations 11

Authors

Ane Marcos-Carcavilla

Jorge H Calvo

Carmen Gonzalez

Katayoun Moazami-Goudarzi

Pascal Laurent

Maud Bertaud

Helene Hayes

Anne E Beattie

Carmen Serrano

Jaber Lyahyai

Inmaculada Martin-Burriel

Magdalena Serrano

Affiliations

Soon will be listed here.

Abstract

Scrapie is a transmissible spongiform encephalopathy in sheep and goats. Susceptibility to this neurodegenerative disease is mainly controlled by point mutations at the PRNP locus. Other genes, apart from PRNP, have been reported to modulate resistance/susceptibility to scrapie. On the basis of several studies in Alzheimer and different transmissible spongiform encephalopathy models, HSP90AA1 was chosen as a putative positional and functional candidate gene that might be involved in the polygenic variance mentioned above. In the present work, the ovine HSP90AA1 gene including the promoter and other regulatory regions has been isolated and characterized. Several sequence polymorphisms have also been identified. FISH-mapping localized the HSP90AA1 gene on ovine chromosome OAR19q24dist, which was confirmed by linkage analysis. This chromosome region has been shown to include a quantitative trait loci (QTL) for scrapie incubation period in sheep. Expression analyses were carried out in spleen and cerebellum samples. No differences in the expression of the HSP90AA1 gene were found in any of these tissues (p > 0.05) between control and infected animal samples. Nevertheless, association analyses revealed that several polymorphisms in the 5' and 3' regions of the HSP90AA1 gene were differentially distributed among animals with different responses to scrapie infection. Thus, results presented here support the hypothesis that HSP90AA1 could be a positional and functional candidate gene modulating the response to scrapie in sheep.

Citing Articles

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In silico analysis of regulatory and structural motifs of the ovine HSP90AA1 gene.

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