Glucose and Lactate Biosensors for Scanning Electrochemical Microscopy Imaging of Single Live Cells

Overview

Journal Anal Chem

Specialty Chemistry

Date 2008 Mar 19

PMID 18345647

Citations 26

Authors

Madalina Ciobanu

Dale E Taylor Jr

Jeremy P Wilburn

David E Cliffel

Affiliations

Soon will be listed here.

Abstract

We have developed glucose and lactate ultramicroelectrode (UME) biosensors based on glucose oxidase and lactate oxidase (with enzymes immobilized onto Pt UMEs by either electropolymerization or casting) for scanning electrochemical microscopy (SECM) and have determined their sensitivity to glucose and lactate, respectively. The results of our evaluations reveal different advantages for sensors constructed by each method: improved sensitivity and shorter manufacturing time for hand-casting, and increased reproducibility for electropolymerization. We have acquired amperometric approach curves (ACs) for each type of manufactured biosensor UME, and these ACs can be used as a means of positioning the UME above a substrate at a known distance. We have used the glucose biosensor UMEs to record profiles of glucose uptake above individual fibroblasts. Likewise, we have employed the lactate biosensor UMEs for recording the lactate production above single cancer cells with the SECM. We also show that oxygen respiration profiles for single cancer cells do not mimic cell topography, but are rather more convoluted, with a higher respiration activity observed at the points where the cell touches the Petri dish. These UME biosensors, along with the application of others already described in the literature, could prove to be powerful tools for mapping metabolic analytes, such as glucose, lactate, and oxygen, in single cancer cells.

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References

Bard A, Li X, Zhan W . Chemically imaging living cells by scanning electrochemical microscopy. Biosens Bioelectron. 2006; 22(4):461-72. DOI: 10.1016/j.bios.2006.04.028. View

Cai C, Liu B, Mirkin M, Frank H, Rusling J . Scanning electrochemical microscopy of living cells. 3. Rhodobacter sphaeroides. Anal Chem. 2002; 74(1):114-9. DOI: 10.1021/ac010945e. View

Anderson A, Weaver A, Cummings P, Quaranta V . Tumor morphology and phenotypic evolution driven by selective pressure from the microenvironment. Cell. 2006; 127(5):905-15. DOI: 10.1016/j.cell.2006.09.042. View

Liebetrau J, Miller H, Baur J, Takacs S, Anupunpisit V, Garris P . Scanning electrochemical microscopy of model neurons: imaging and real-time detection of morphological changes. Anal Chem. 2003; 75(3):563-71. DOI: 10.1021/ac026166v. View

Mauzeroll J, Bard A, Owhadian O, Monks T . Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy. Proc Natl Acad Sci U S A. 2004; 101(51):17582-7. PMC: 539751. DOI: 10.1073/pnas.0407613101. View

Liu B, Rotenberg S, Mirkin M . Scanning electrochemical microscopy of living cells: different redox activities of nonmetastatic and metastatic human breast cells. Proc Natl Acad Sci U S A. 2000; 97(18):9855-60. PMC: 27604. DOI: 10.1073/pnas.97.18.9855. View

Devadoss A, Burgess J . Steady-state detection of cholesterol contained in the plasma membrane of a single cell using lipid bilayer-modified microelectrodes incorporating cholesterol oxidase. J Am Chem Soc. 2004; 126(33):10214-5. DOI: 10.1021/ja047856e. View

Kurulugama R, Wipf D, Takacs S, Pongmayteegul S, Garris P, Baur J . Scanning electrochemical microscopy of model neurons: constant distance imaging. Anal Chem. 2005; 77(4):1111-7. DOI: 10.1021/ac048571n. View

Bhujwalla Z, Artemov D, Aboagye E, Ackerstaff E, Gillies R, Natarajan K . The physiological environment in cancer vascularization, invasion and metastasis. Novartis Found Symp. 2001; 240:23-38; discussion 38-45, 152-3. DOI: 10.1002/0470868716.ch3. View

10.

Kueng A, Kranz C, Lugstein A, Bertagnolli E, Mizaikoff B . AFM-tip-integrated amperometric microbiosensors: high-resolution imaging of membrane transport. Angew Chem Int Ed Engl. 2005; 44(22):3419-22. DOI: 10.1002/anie.200461556. View

11.

Hengstenberg A, Kranz C, Schuhmann W . Facilitated tip-positioning and applications of non-electrode tips in scanning electrochemical microscopy using a shear force based constant-distance mode. Chemistry. 2000; 6(9):1547-54. DOI: 10.1002/(sici)1521-3765(20000502)6:9<1547::aid-chem1547>3.3.co;2-3. View

12.

Semenza G, Artemov D, Bedi A, Bhujwalla Z, Chiles K, Feldser D . 'The metabolism of tumours': 70 years later. Novartis Found Symp. 2001; 240:251-60; discussion 260-4. View

13.

Santner S, Dawson P, Tait L, SOULE H, Eliason J, Mohamed A . Malignant MCF10CA1 cell lines derived from premalignant human breast epithelial MCF10AT cells. Breast Cancer Res Treat. 2001; 65(2):101-10. DOI: 10.1023/a:1006461422273. View

14.

Lindner E, Buck R . Microfabricated potentiometric electrodes and their in vivo applications. Anal Chem. 2000; 72(9):336A-345A. DOI: 10.1021/ac002805v. View

15.

Anderson A . A hybrid mathematical model of solid tumour invasion: the importance of cell adhesion. Math Med Biol. 2005; 22(2):163-86. DOI: 10.1093/imammb/dqi005. View

16.

Yao T, Yano T, Nanjyo Y, Nishino H . Simultaneous determination of glucose and L-lactate in rat brain by an electrochemical in vivo flow-injection system with an on-line microdialysis sampling. Anal Sci. 2003; 19(1):61-5. DOI: 10.2116/analsci.19.61. View

17.

Feng W, Rotenberg S, Mirkin M . Scanning electrochemical microscopy of living cells. 5. Imaging of fields of normal and metastatic human breast cells. Anal Chem. 2003; 75(16):4148-54. DOI: 10.1021/ac0343127. View

18.

Macpherson J, Unwin P . Noncontact electrochemical imaging with combined scanning electrochemical atomic force microscopy. Anal Chem. 2001; 73(3):550-7. DOI: 10.1021/ac001072b. View

19.

Jiang D, Devadoss A, Palencsar M, Fang D, White N, Kelley T . Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells. J Am Chem Soc. 2007; 129(37):11352-3. DOI: 10.1021/ja074373c. View

20.

Hengstenberg A, Blochl A, Dietzel I, Schuhmann W . Spatially Resolved Detection of Neurotransmitter Secretion from Individual Cells by Means of Scanning Electrochemical Microscopy. Angew Chem Int Ed Engl. 2001; 40(5):905-908. View