Comparative Proteomics Analysis of Cerebrospinal Fluid of Patients with Guillain-Barré Syndrome

Overview

Journal Cell Mol Neurobiol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Neurology

Date 2008 Mar 18

PMID 18343991

Citations 23

Authors

Yin-Rong Yang

Shi-Lian Liu

Zhao-Yu Qin

Fu-Jun Liu

Yan-Jiang Qin

Shu-Mei Bai

Zhe-Yu Chen

Affiliations

Soon will be listed here.

Abstract

To better understand the pathophysiologic mechanisms underlying Guillain-Barré syndrome (GBS), Comparative proteomic analysis of cerebrospinal fluid (CSF) between patients with GBS (the experiment group) and control subjects suffering from other neurological disorders (the control group) was carried out using two-dimensional gel electrophoresis (2-DE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and database searching to determine abnormal CSF proteins in GBS patients. Image analysis of 2-DE gels silver stained revealed that 10 protein spots showed significant differential expression between the two groups of CSF samples. The expression of cystatin C, transthyretin, apolipoprotein E and heat shock protein 70 were decreased. However, haptoglobin, alpha-1-antitrypsin, apolipoprotein A-IV and neurofilaments were elevated. The subsequent ELISA measured the concentration of cystatin C and confirmed the result of the proteomic analysis. These identified proteins may be involved in the pathophysiological process of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.

Citing Articles

Current Biomarker Strategies in Autoimmune Neuromuscular Diseases.

Oeztuerk M, Henes A, Schroeter C, Nelke C, Quint P, Theissen L Cells. 2023; 12(20).

PMID: 37887300 PMC: 10605022. DOI: 10.3390/cells12202456.

CSF Diagnostics: A Potentially Valuable Tool in Neurodegenerative and Inflammatory Disorders Involving Motor Neurons: A Review.

Krause K, Wulf M, Sommer P, Barkovits K, Vorgerd M, Marcus K Diagnostics (Basel). 2021; 11(9).

PMID: 34573864 PMC: 8470638. DOI: 10.3390/diagnostics11091522.

Nusinersen Modulates Proteomics Profiles of Cerebrospinal Fluid in Spinal Muscular Atrophy Type 1 Patients.

Bianchi L, Sframeli M, Vantaggiato L, Vita G, Ciranni A, Polito F Int J Mol Sci. 2021; 22(9).

PMID: 33919289 PMC: 8122268. DOI: 10.3390/ijms22094329.

The Neuroimmunology of Guillain-Barré Syndrome and the Potential Role of an Aging Immune System.

Hagen K, Ousman S Front Aging Neurosci. 2021; 12:613628.

PMID: 33584245 PMC: 7873882. DOI: 10.3389/fnagi.2020.613628.

Thyroid Hormone Distributor Proteins During Development in Vertebrates.

Rabah S, Gowan I, Pagnin M, Osman N, Richardson S Front Endocrinol (Lausanne). 2019; 10:506.

PMID: 31440205 PMC: 6694296. DOI: 10.3389/fendo.2019.00506.

References

Yu S, Duan R, Chen Z, Quezada H, Bao L, Nennesmo I . Increased susceptibility to experimental autoimmune neuritis after upregulation of the autoreactive T cell response to peripheral myelin antigen in apolipoprotein E-deficient mice. J Neuropathol Exp Neurol. 2004; 63(2):120-8. DOI: 10.1093/jnen/63.2.120. View

Manetto V, Sternberger N, Perry G, STERNBERGER L, Gambetti P . Phosphorylation of neurofilaments is altered in amyotrophic lateral sclerosis. J Neuropathol Exp Neurol. 1988; 47(6):642-53. DOI: 10.1097/00005072-198811000-00007. View

Volchkova E, Pak S, Malov V, Umbetova K . [Changes in the levels of acute phase proteins in viral hepatitis]. Ter Arkh. 2001; 72(11):18-21. View

Hochstrasser D, PATCHORNIK A, Merril C . Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining. Anal Biochem. 1988; 173(2):412-23. DOI: 10.1016/0003-2697(88)90208-4. View

Serot J, Christmann D, DUBOST T, Couturier M . Cerebrospinal fluid transthyretin: aging and late onset Alzheimer's disease. J Neurol Neurosurg Psychiatry. 1997; 63(4):506-8. PMC: 2169793. DOI: 10.1136/jnnp.63.4.506. View

Wagner L, Gessl A, Parzer S, Base W, Waldhausl W, Pasternack M . Haptoglobin phenotyping by newly developed monoclonal antibodies. Demonstration of haptoglobin uptake into peripheral blood neutrophils and monocytes. J Immunol. 1996; 156(5):1989-96. View

Rosengren L, Karlsson J, Karlsson J, Persson L, Wikkelso C . Patients with amyotrophic lateral sclerosis and other neurodegenerative diseases have increased levels of neurofilament protein in CSF. J Neurochem. 1996; 67(5):2013-8. DOI: 10.1046/j.1471-4159.1996.67052013.x. View

Sotelo-Silveira J, Calliari A, Kun A, Benech J, Sanguinetti C, Chalar C . Neurofilament mRNAs are present and translated in the normal and severed sciatic nerve. J Neurosci Res. 2000; 62(1):65-74. DOI: 10.1002/1097-4547(20001001)62:1<65::AID-JNR7>3.0.CO;2-Z. View

Jin T, Hu L, Chang M, Wu J, Winblad B, Zhu J . Proteomic identification of potential protein markers in cerebrospinal fluid of GBS patients. Eur J Neurol. 2007; 14(5):563-8. DOI: 10.1111/j.1468-1331.2007.01761.x. View

10.

Laurat E, Poirier B, Tupin E, Caligiuri G, Hansson G, Bariety J . In vivo downregulation of T helper cell 1 immune responses reduces atherogenesis in apolipoprotein E-knockout mice. Circulation. 2001; 104(2):197-202. DOI: 10.1161/01.cir.104.2.197. View

11.

Yasmin M, Aziz B, Nazim M, Madhavan R . Prealbumin rather than albumin is a more sensitive indicator of acute liver disease. Malays J Pathol. 1993; 15(2):147-50. View

12.

Asbury A, Cornblath D . Assessment of current diagnostic criteria for Guillain-Barré syndrome. Ann Neurol. 1990; 27 Suppl:S21-4. DOI: 10.1002/ana.410270707. View

13.

Langner C, Birkenmeier E, Roth K, Bronson R, Gordon J . Characterization of the peripheral neuropathy in neonatal and adult mice that are homozygous for the fatty liver dystrophy (fld) mutation. J Biol Chem. 1991; 266(18):11955-64. View

14.

Berkova N, Gilbert C, Goupil S, Yan J, Korobko V, Naccache P . TNF-induced haptoglobin release from human neutrophils: pivotal role of the TNF p55 receptor. J Immunol. 1999; 162(10):6226-32. View

15.

Shen P, Howlett G . Alteration in rat apolipoprotein C-III gene expression and lipoprotein composition during inflammation. Inflammation. 1993; 17(2):153-66. DOI: 10.1007/BF00916102. View

16.

Goldman J, Yen S, Chiu F, Peress N . Lewy bodies of Parkinson's disease contain neurofilament antigens. Science. 1983; 221(4615):1082-4. DOI: 10.1126/science.6308771. View

17.

Sanchez J, Rouge V, Pisteur M, Ravier F, Tonella L, Moosmayer M . Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients. Electrophoresis. 1997; 18(3-4):324-7. DOI: 10.1002/elps.1150180305. View

18.

Lehmensiek V, Sussmuth S, Brettschneider J, Tauscher G, Felk S, Gillardon F . Proteome analysis of cerebrospinal fluid in Guillain-Barré syndrome (GBS). J Neuroimmunol. 2007; 185(1-2):190-4. DOI: 10.1016/j.jneuroim.2007.01.022. View

19.

Ranganathan S, Williams E, Ganchev P, Gopalakrishnan V, Lacomis D, Urbinelli L . Proteomic profiling of cerebrospinal fluid identifies biomarkers for amyotrophic lateral sclerosis. J Neurochem. 2005; 95(5):1461-71. PMC: 1540444. DOI: 10.1111/j.1471-4159.2005.03478.x. View

20.

Ropper A . The Guillain-Barré syndrome. N Engl J Med. 1992; 326(17):1130-6. DOI: 10.1056/NEJM199204233261706. View