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Evaluation of 16S RRNA Gene-based PCR Assays for Genus-level Identification of Helicobacter Species

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2008 Mar 14
PMID 18337392
Citations 8
Authors
H Moyaert
F Pasmans
R Ducatelle
F Haesebrouck
M Baele
Affiliations
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Abstract

The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.

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