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Expression, Purification, Crystallization and Preliminary X-ray Characterization of Two Crystal Forms of Stationary-phase Survival E Protein from Campylobacter Jejuni

Overview
Journal Acta Crystallogr Sect F Struct Biol Cryst Commun
Date 2008 Mar 8
PMID 18323612
Citations 1
Authors
A M D Goncalves
A T Rego
M Thomaz
F J Enguita
M A Carrondo
Affiliations
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Abstract

Survival E (SurE) protein from Campylobacter jejuni, a Gram-negative mesophile, has been overexpressed in Escherichia coli as a soluble protein, successfully purified and crystallized in two distinct crystal forms. The first form belongs to space group P2(1)2(1)2(1), with a tetramer in the asymmetric unit and unit-cell parameters a = 80.5, b = 119.0, c = 135.3 A. The second form belongs to space group C2, with unit-cell parameters a = 121.4, b = 47.1, c = 97.8 A, and contains a dimer in the asymmetric unit. Diffraction data have been collected from these crystal forms to 2.5 and 2.95 A resolution, respectively.

Citing Articles

Crystallization and preliminary X-ray analysis of stationary phase survival protein E (SurE) from Xylella fastidiosa in two crystal forms.

Reis M, Saraiva A, Dos Santos M, de Souza A, Aparicio R Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012; 68(Pt 4):464-7.

PMID: 22505421 PMC: 3325821. DOI: 10.1107/S1744309112007129.

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