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Sequence Analysis of a CDNA Encoding a Group 3 LEA MRNA Inducible by ABA or Dehydration Stress in Wheat

Overview
Journal Plant Mol Biol
Date 1991 Jun 1
PMID 1830822
Citations 28
Authors
J Curry
C F Morris
M K Walker-Simmons
Affiliations
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Abstract

A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475-486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23,000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.

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References
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