A Novel and Highly Sensitive Resonance Scattering Spectral Assay for Horseradish Peroxidase Using Cationic Surfactant

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2008 Feb 28

PMID 18302009

Authors

Ai-hui Liang

Bo Jiang

Ji Ma

Zhi-Liang Jiang

Ji-Shun Li

Affiliations

Soon will be listed here.

Abstract

A novel and ultrasensitive resonance scattering (RS) spectral assay was proposed for detection of horseradish peroxidase (HRP) activity. It was based on that the HRP strongly catalyze H2O2 oxidation of excess I- to form I3-, the resulting I3- combined with four cationic surfactant (CS), including tetradecyl pyridinium bromide (TPB), cetyltrimethyl ammonium bromide (CTMA), cetylpyridinium chloride (CPCM) and tetradecyl dimethylbenzyl ammonium chloride (TDMBA) to produce association particles (TPB-I3)n, (CTMA-I3)m, (CPCM-I3)l and (TDMBA-I3)k, which exhibit a strongest resonance scattering peak at 478, 423, 538 and 491 nm, respectively. For the four systems of TPB, CPCM, CTMBA and TDMBA, the HRP activity determined was in the linear range of 0.004-5.6, 0.04-3.2, 0.04-8.0, 0.08-8.0 ng/mL, with a detection limit of 0.0034, 0.040, 0.033, 0.016 ng/mL, respectively. The TPB resonance scattering spectral assay was best and has been applied to the analysis of HRP in real samples, with satisfactory results.

References

Liu S, Luo H, Li N, Liu Z, Zheng W . Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes. Anal Chem. 2001; 73(16):3907-14. DOI: 10.1021/ac001454h. View

Wei Y, Yan H . [A novel catalytic spectrophotometric determination for horseradish peroxidase]. Guang Pu Xue Yu Guang Pu Fen Xi. 2003; 21(5):704-6. View

Pan H, Liang F, Mao C, Zhu J, Chen H . Highly luminescent zinc(II)-bis(8-hydroxyquinoline) complex nanorods: sonochemical synthesis, characterizations, and protein sensing. J Phys Chem B. 2007; 111(20):5767-72. DOI: 10.1021/jp0703049. View

Molaei Rad A, Ghourchian H, Moosavi-Movahedi A, Hong J, Nazari K . Spectrophotometric assay for horseradish peroxidase activity based on pyrocatechol-aniline coupling hydrogen donor. Anal Biochem. 2007; 362(1):38-43. DOI: 10.1016/j.ab.2006.11.035. View

Huang Q, Selig H, Weber Jr W . Peroxidase-catalyzed oxidative coupling of phenols in the presence of geosorbents: rates of non-extractable product formation. Environ Sci Technol. 2002; 36(4):596-602. DOI: 10.1021/es010512t. View

Hou M, Sun S, Jiang Z . A new and selective and sensitive nanogold-labeled immunoresoance scattering spectral assay for trace prealbumin. Talanta. 2008; 72(2):463-7. DOI: 10.1016/j.talanta.2006.11.016. View

Jiang Z, Zhou S, Liang A, Kang C, He X . Resonance scattering effect of rhodamine dye association nanoparticles and its application to respective determination of trace ClO2 and Cl2. Environ Sci Technol. 2006; 40(13):4286-91. DOI: 10.1021/es051949u. View

Siddiqi I . An electrochemical assay system for peroxidase and peroxidase-couplers reactions based on a fluoride ion-selective electrode. Clin Chem. 1982; 28(9):1962-7. View

Fanjul-Bolado P, Gonzalez-Garcia M, Costa-Garcia A . Voltammetric determination of alkaline phosphatase and horseradish peroxidase activity using 3-indoxyl phosphate as substrate Application to enzyme immunoassay. Talanta. 2008; 64(2):452-7. DOI: 10.1016/j.talanta.2004.03.003. View

10.

Luo H, Li N, Liu S . Resonance Rayleigh scattering study of interaction of hyaluronic acid with ethyl violet dye and its analytical application. Biosens Bioelectron. 2005; 21(7):1186-94. DOI: 10.1016/j.bios.2005.04.018. View

11.

Yao H, Li N, Xu S, Xu J, Zhu J, Chen H . Electrochemical study of a new methylene blue/silicon oxide nanocomposition mediator and its application for stable biosensor of hydrogen peroxide. Biosens Bioelectron. 2005; 21(2):372-7. DOI: 10.1016/j.bios.2004.08.051. View

12.

Du B, Li Z, Liu C . One-step homogeneous detection of DNA hybridization with gold nanoparticle probes by using a linear light-scattering technique. Angew Chem Int Ed Engl. 2006; 45(47):8022-5. DOI: 10.1002/anie.200603331. View

13.

Han Z, Qi L, Shen G, Liu W, Chen Y . Determination of chromium(VI) by surface plasmon field-enhanced resonance light scattering. Anal Chem. 2007; 79(15):5862-8. DOI: 10.1021/ac062453d. View

14.

Wu L, Li Y, Huang C, Zhang Q . Visual detection of Sudan dyes based on the plasmon resonance light scattering signals of silver nanoparticles. Anal Chem. 2006; 78(15):5570-7. DOI: 10.1021/ac0603577. View

15.

Huang Q, Weber Jr W . Transformation and removal of bisphenol A from aqueous phase via peroxidase mediated oxidative coupling reactions: efficacy, products, and pathways. Environ Sci Technol. 2005; 39(16):6029-36. DOI: 10.1021/es050036x. View

16.

Jiang Z, Sun S, Liang A, Huang W, Qin A . Gold-labeled nanoparticle-based immunoresonance scattering spectral assay for trace apolipoprotein AI and apolipoprotein B. Clin Chem. 2006; 52(7):1389-94. DOI: 10.1373/clinchem.2005.061176. View