» Articles » PMID: 18290669

Subsurface Biomolecular Imaging of Streptomyces Coelicolor Using Secondary Ion Mass Spectrometry

Overview
Journal Anal Chem
Specialty Chemistry
Date 2008 Feb 23
PMID 18290669
Citations 21
Authors
Affiliations
Soon will be listed here.
Abstract

Imaging using time-of-flight secondary ion mass spectrometry (TOF-SIMS) with buckministerfullerene (C(60)) primary ions offers the possibility of mapping the chemical distribution of molecular species from biological surfaces. Here we demonstrate the capability of the technique to provide biomolecular information from the cell surface as well as from within the surface, as illustrated with the distribution of two antibiotics in Streptomyces coelicolor (a mycelial bacterium). Differential production of the two pigmented antibiotics under salt-stressed and normal conditions in submerged cultivations could be detected from the TOF-SIMS spectra of the bacteria, demonstrating the potential of the technique in studying microbial physiology. Although both the antibiotics were detected on the cell surface, sputter etching with C(60)(+) revealed the spectral features of only one of the antibiotics within the cells. Exploratory analysis of the images using principal component analysis assisted in analyzing the spectral information with respect to peak contributions and their spatial distributions. The technique allows the study of not only lateral but also the depthwise distribution of biomolecules, uniquely enabling exploration of the processes within biological systems with minimal system intervention and with little a priori biochemical knowledge of relevance.

Citing Articles

Development of Peptide Identification System for ToF-SIMS Spectra Using Supervised Machine Learning.

Aoyagi S, Fujita M, Itoh H, Itoh H, Nagatomi T, Okamoto M J Am Soc Mass Spectrom. 2024; 35(12):3057-3062.

PMID: 39395019 PMC: 11623169. DOI: 10.1021/jasms.4c00310.


Single Cell mass spectrometry: Towards quantification of small molecules in individual cells.

Lan Y, Zou Z, Yang Z Trends Analyt Chem. 2024; 174.

PMID: 39391010 PMC: 11465888. DOI: 10.1016/j.trac.2024.117657.


PCA, PC-CVA, and Random Forest of GCIB-SIMS Data for the Elucidation of Bacterial Envelope Differences in Antibiotic Resistance Research.

Fransson A, Dimovska Nilsson K, Henderson A, Farewell A, Fletcher J Anal Chem. 2024; 96(35):14168-14177.

PMID: 39163401 PMC: 11375623. DOI: 10.1021/acs.analchem.4c02093.


Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.

Parmar D, Rosado-Rosa J, Shrout J, Sweedler J Methods. 2024; 224:21-34.

PMID: 38295894 PMC: 11149699. DOI: 10.1016/j.ymeth.2024.01.014.


Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry.

Robertson S, Soukarieh F, White T, Camara M, Romero M, Griffiths R Anal Chem. 2023; 95(11):5079-5086.

PMID: 36881460 PMC: 10034741. DOI: 10.1021/acs.analchem.2c05703.