An Additional Function for Bacteriophage Lambda Rex: the RexB Product Prevents Degradation of the Lambda O Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Jun 1

PMID 1828888

Citations 10

Authors

H Engelberg-Kulka

Affiliations

Soon will be listed here.

Abstract

The rex operon of bacteriophage lambda excludes the development of several unrelated bacteriophages. Here we present an additional lambda rexB function: it prevents degradation of the short-lived protein lambda O known to be involved in lambda DNA replication. We have shown that it is the product of rexB that is responsible for the stabilization of lambda O: when a nonsense mutation is present in rexB, lambda O protein is labile; suppression of the mutation by the corresponding nonsense suppressor causes partial restabilization of lambda O. lambda rexB also stabilizes lambda O in trans. We discuss our results in relation to the function of rexB in lambda DNA replication and its role in the protein degradation pathways of bacteriophage lambda.

Citing Articles

The crystal structure of bacteriophage λ RexA provides novel insights into the DNA binding properties of Rex-like phage exclusion proteins.

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Bacteriophage λ RexA and RexB functions assist the transition from lysogeny to lytic growth.

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Stationary phase-like properties of the bacteriophage lambda Rex exclusion phenotype.

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rexB of bacteriophage lambda is an anti-cell death gene.

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Regulation of proteolysis of the stationary-phase sigma factor RpoS.

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References

Crick F, Barnett L, Brenner S, WATTS-TOBIN R . General nature of the genetic code for proteins. Nature. 1961; 192:1227-32. DOI: 10.1038/1921227a0. View

Howard B . Phage lambda mutants deficient in r-II exclusion. Science. 1967; 158(3808):1588-9. DOI: 10.1126/science.158.3808.1588. View

Cheng H, Muhlrad P, Hoyt M, Echols H . Cleavage of the cII protein of phage lambda by purified HflA protease: control of the switch between lysis and lysogeny. Proc Natl Acad Sci U S A. 1988; 85(21):7882-6. PMC: 282301. DOI: 10.1073/pnas.85.21.7882. View

Martinez E, Bartolome B, de la Cruz F . pACYC184-derived cloning vectors containing the multiple cloning site and lacZ alpha reporter gene of pUC8/9 and pUC18/19 plasmids. Gene. 1988; 68(1):159-62. DOI: 10.1016/0378-1119(88)90608-7. View

Shinedling S, Parma D, Gold L . Wild-type bacteriophage T4 is restricted by the lambda rex genes. J Virol. 1987; 61(12):3790-4. PMC: 255994. DOI: 10.1128/JVI.61.12.3790-3794.1987. View

Banuett F, Hoyt M, McFarlane L, Echols H, Herskowitz I . hflB, a new Escherichia coli locus regulating lysogeny and the level of bacteriophage lambda cII protein. J Mol Biol. 1986; 187(2):213-24. DOI: 10.1016/0022-2836(86)90229-9. View

Alfano C, McMacken R . Heat shock protein-mediated disassembly of nucleoprotein structures is required for the initiation of bacteriophage lambda DNA replication. J Biol Chem. 1989; 264(18):10709-18. View

Snyder L, McWilliams K . The rex genes of bacteriophage lambda can inhibit cell function without phage superinfection. Gene. 1989; 81(1):17-24. DOI: 10.1016/0378-1119(89)90332-6. View

DODSON M, McMacken R, Echols H . Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda. Protein association and disassociation reactions responsible for localized initiation of replication. J Biol Chem. 1989; 264(18):10719-25. View

10.

Herskowitz I, Hagen D . The lysis-lysogeny decision of phage lambda: explicit programming and responsiveness. Annu Rev Genet. 1980; 14:399-445. DOI: 10.1146/annurev.ge.14.120180.002151. View

11.

Raftery L, Egan J, Cline S, Yarus M . Defined set of cloned termination suppressors: in vivo activity of isogenetic UAG, UAA, and UGA suppressor tRNAs. J Bacteriol. 1984; 158(3):849-59. PMC: 215519. DOI: 10.1128/jb.158.3.849-859.1984. View

12.

Benzer S . FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1955; 41(6):344-54. PMC: 528093. DOI: 10.1073/pnas.41.6.344. View

13.

Rattray A, Altuvia S, Mahajna G, Oppenheim A, Gottesman M . Control of bacteriophage lambda CII activity by bacteriophage and host functions. J Bacteriol. 1984; 159(1):238-42. PMC: 215619. DOI: 10.1128/jb.159.1.238-242.1984. View

14.

Simatake H, Rosenberg M . Purified lambda regulatory protein cII positively activates promoters for lysogenic development. Nature. 1981; 292(5819):128-32. DOI: 10.1038/292128a0. View

15.

Landsmann J, Kroger M, HOBOM G . The rex region of bacteriophage lambda: two genes under three-way control. Gene. 1982; 20(1):11-24. DOI: 10.1016/0378-1119(82)90083-x. View

16.

Wold M, Mallory J, Roberts J, Lebowitz J, McMacken R . Initiation of bacteriophage lambda DNA replication in vitro with purified lambda replication proteins. Proc Natl Acad Sci U S A. 1982; 79(20):6176-80. PMC: 347082. DOI: 10.1073/pnas.79.20.6176. View

17.

Matz K, Schmandt M, Gussin G . The rex gene of bacteriophage lambda is really two genes. Genetics. 1982; 102(3):319-27. PMC: 1201943. DOI: 10.1093/genetics/102.3.319. View

18.

Hoyt M, Knight D, Das A, MILLER H, Echols H . Control of phage lambda development by stability and synthesis of cII protein: role of the viral cIII and host hflA, himA and himD genes. Cell. 1982; 31(3 Pt 2):565-73. DOI: 10.1016/0092-8674(82)90312-9. View

19.

Kuypers B, REISER W, Klein A . Cloning of the replication gene O of E. coli bacteriophage lambda and its expression under the control of the lac promoter. Gene. 1980; 10(3):195-203. DOI: 10.1016/0378-1119(80)90049-9. View

20.

Gottesman S, Gottesman M, Shaw J, Pearson M . Protein degradation in E. coli: the lon mutation and bacteriophage lambda N and cII protein stability. Cell. 1981; 24(1):225-33. DOI: 10.1016/0092-8674(81)90518-3. View