» Articles » PMID: 18288539

Comparison of Electrophoretic Protein Profiles from Sheep and Goat Parotid Saliva

Overview
Journal J Chem Ecol
Publisher Springer
Date 2008 Feb 22
PMID 18288539
Citations 5
Authors
Affiliations
Soon will be listed here.
Abstract

Saliva provides a medium for short-term adaptation to changes in diet composition, namely, the presence of plant secondary metabolites. Salivary proteins have biological functions that have particular influence on oral homeostasis, taste, and digestive function. Some salivary proteins, such as proline-rich proteins, are present in browsers but absent in grazers. Despite the significance of salivary proteins, their expression patterns in many herbivores are unknown. We investigated the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile of parotid salivary proteins from two domesticated species, one a grazer, the sheep, Ovis aries, and the other a mixed feeder, the goat, Capra hircus, both fed on the same conventional diet. With 12.5% polyacrylamide linear gels, we observed uniform patterns of salivary proteins within the two species. In the goat profile, 21 major bands were observed, and 19 in the sheep profile. Each band was subjected to peptide mass fingerprinting for purposes of identification, allowing for 16 successful protein identifications. Marked differences were observed between the species in the region of 25-35 kDa molecular weights: one band was present in significantly different intensities; three bands were present only in goats; and one band was present only in sheep. This is the first report of a comparison of the protein salivary composition of sheep and goats and suggests that future research should be conducted to reveal a physiological function for salivary proteins related to the differences in feeding behavior of these species.

Citing Articles

Are there phylogenetic differences in salivary tannin-binding proteins between browsers and grazers, and ruminants and hindgut fermenters?.

Ward D, Schmitt M, Shrader A Ecol Evol. 2020; 10(19):10426-10439.

PMID: 33072270 PMC: 7548203. DOI: 10.1002/ece3.6698.


Can Agro-Industrial By-Products Rich in Polyphenols be Advantageously Used in the Feeding and Nutrition of Dairy Small Ruminants?.

Correddu F, Lunesu M, Buffa G, Atzori A, Nudda A, Battacone G Animals (Basel). 2020; 10(1).

PMID: 31947543 PMC: 7022336. DOI: 10.3390/ani10010131.


The effect of tannins on Mediterranean ruminant ingestive behavior: the role of the oral cavity.

Lamy E, Rawel H, Schweigert F, Capela E Silva F, Ferreira A, Costa A Molecules. 2011; 16(4):2766-84.

PMID: 21441875 PMC: 6260606. DOI: 10.3390/molecules16042766.


Changes in mouse whole saliva soluble proteome induced by tannin-enriched diet.

Lamy E, Graca G, da Costa G, Franco C, Capela E Silva F, Baptista E Proteome Sci. 2010; 8:65.

PMID: 21159160 PMC: 3018447. DOI: 10.1186/1477-5956-8-65.


No major role for binding by salivary proteins as a defense against dietary tannins in Mediterranean goats.

Hanovice-Ziony M, Gollop N, Landau S, Ungar E, Muklada H, Glasser T J Chem Ecol. 2010; 36(7):736-43.

PMID: 20559693 DOI: 10.1007/s10886-010-9809-z.

References
1.
Kimoto M, Kishino M, Yura Y, Ogawa Y . A role of salivary carbonic anhydrase VI in dental plaque. Arch Oral Biol. 2005; 51(2):117-22. DOI: 10.1016/j.archoralbio.2005.04.007. View

2.
Henkin R, Martin B, Agarwal R . Decreased parotid saliva gustin/carbonic anhydrase VI secretion: an enzyme disorder manifested by gustatory and olfactory dysfunction. Am J Med Sci. 2000; 318(6):380-91. DOI: 10.1097/00000441-199912000-00005. View

3.
Walz A, Stuhler K, Wattenberg A, Hawranke E, Meyer H, Schmalz G . Proteome analysis of glandular parotid and submandibular-sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis. Proteomics. 2006; 6(5):1631-9. DOI: 10.1002/pmic.200500125. View

4.
Ekstrom J, Marshall T, Tobin G, Williams K . Electrophoretic analysis of rat parotid salivary protein composition: investigation of the parasympathetic atropine-resistant secretion. Acta Physiol Scand. 1996; 156(1):75-9. DOI: 10.1046/j.1365-201X.1996.428149000.x. View

5.
Wilmarth P, Riviere M, Rustvold D, Lauten J, Madden T, David L . Two-dimensional liquid chromatography study of the human whole saliva proteome. J Proteome Res. 2004; 3(5):1017-23. DOI: 10.1021/pr049911o. View