Genetic Analysis of the FlaA Locus of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Jun 1

PMID 1828466

Citations 3

Authors

P M Hauser

W D Crabb

M G Fiora

F Scoffone

A Galizzi

Affiliations

Soon will be listed here.

Abstract

We isolated two clones of recombinant lambda bacteriophage with overlapping inserts of Bacillus subtilis chromosomal DNA corresponding to part of the flaA locus. The flaA4 and flaA15 mutations were localized on the physical map by marker rescue experiments. The flaA locus and the flaB (sigD) gene were mapped in transduction crosses, and the order glnA polC flaB flaA was determined. FlaB was linked to polC in transformation crosses.

Citing Articles

Temporal regulation of sigD from Bacillus subtilis depends on a minor promoter in front of the gene.

Allmansberger R J Bacteriol. 1997; 179(20):6531-5.

PMID: 9335309 PMC: 179576. DOI: 10.1128/jb.179.20.6531-6535.1997.

Coupling of flagellin gene transcription to flagellar assembly in Bacillus subtilis.

Barilla D, Caramori T, Galizzi A J Bacteriol. 1994; 176(15):4558-64.

PMID: 8045886 PMC: 196275. DOI: 10.1128/jb.176.15.4558-4564.1994.

The flaA locus of Bacillus subtilis is part of a large operon coding for flagellar structures, motility functions, and an ATPase-like polypeptide.

Albertini A, Caramori T, Crabb W, Scoffone F, Galizzi A J Bacteriol. 1991; 173(11):3573-9.

PMID: 1828465 PMC: 207974. DOI: 10.1128/jb.173.11.3573-3579.1991.

References

Davis B, MINGIOLI E . Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950; 60(1):17-28. PMC: 385836. DOI: 10.1128/jb.60.1.17-28.1950. View

Hoch J, Barat M, Anagnostopoulos C . Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967; 93(6):1925-37. PMC: 276712. DOI: 10.1128/jb.93.6.1925-1937.1967. View

Albertini A, Caramori T, Crabb W, Scoffone F, Galizzi A . The flaA locus of Bacillus subtilis is part of a large operon coding for flagellar structures, motility functions, and an ATPase-like polypeptide. J Bacteriol. 1991; 173(11):3573-9. PMC: 207974. DOI: 10.1128/jb.173.11.3573-3579.1991. View

Gianni M, Galizzi A . Isolation of genes preferentially expressed during Bacillis subtilis spore outgrowth. J Bacteriol. 1986; 165(1):123-32. PMC: 214379. DOI: 10.1128/jb.165.1.123-132.1986. View

Akamatsu T, Sekiguchi J . Genetic mapping by means of protoplast fusion in Bacillus subtilis. Mol Gen Genet. 1987; 208(1-2):254-62. DOI: 10.1007/BF00330451. View

Sekiguchi J, Ezaki B, Kodama K, Akamatsu T . Molecular cloning of a gene affecting the autolysin level and flagellation in Bacillus subtilis. J Gen Microbiol. 1988; 134(6):1611-21. DOI: 10.1099/00221287-134-6-1611. View

Pooley H, KARAMATA D . Genetic analysis of autolysin-deficient and flagellaless mutants of Bacillus subtilis. J Bacteriol. 1984; 160(3):1123-9. PMC: 215829. DOI: 10.1128/jb.160.3.1123-1129.1984. View

Ordal G, Parker H, Kirby J . Complementation and characterization of chemotaxis mutants of Bacillus subtilis. J Bacteriol. 1985; 164(2):802-10. PMC: 214322. DOI: 10.1128/jb.164.2.802-810.1985. View

Marquez L, Helmann J, Ferrari E, Parker H, Ordal G, CHAMBERLIN M . Studies of sigma D-dependent functions in Bacillus subtilis. J Bacteriol. 1990; 172(6):3435-43. PMC: 209155. DOI: 10.1128/jb.172.6.3435-3443.1990. View

10.

Zuberi A, Ying C, Weinreich M, Ordal G . Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis. J Bacteriol. 1990; 172(4):1870-6. PMC: 208681. DOI: 10.1128/jb.172.4.1870-1876.1990. View

11.

FERRARI F, Nguyen A, Lang D, Hoch J . Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol. 1983; 154(3):1513-5. PMC: 217638. DOI: 10.1128/jb.154.3.1513-1515.1983. View

12.

Ordal G, Nettleton D, Hoch J . Genetics of Bacillus subtilis chemotaxis: isolation and mapping of mutations and cloning of chemotaxis genes. J Bacteriol. 1983; 154(3):1088-97. PMC: 217578. DOI: 10.1128/jb.154.3.1088-1097.1983. View

13.

Shioi J, Matsuura S, Imae Y . Quantitative measurements of proton motive force and motility in Bacillus subtilis. J Bacteriol. 1980; 144(3):891-7. PMC: 294750. DOI: 10.1128/jb.144.3.891-897.1980. View

14.

Khan S, Macnab R . Proton chemical potential, proton electrical potential and bacterial motility. J Mol Biol. 1980; 138(3):599-614. DOI: 10.1016/s0022-2836(80)80019-2. View

15.

Zuberi A, Ying C, Parker H, Ordal G . Transposon Tn917lacZ mutagenesis of Bacillus subtilis: identification of two new loci required for motility and chemotaxis. J Bacteriol. 1990; 172(12):6841-8. PMC: 210801. DOI: 10.1128/jb.172.12.6841-6848.1990. View