Interleukin-1 Receptor-associated Kinase (IRAK) -1-mediated NF-kappaB Activation Requires Cytosolic and Nuclear Activity

Interleukin-1 receptor-associated kinase (IRAK) -1 plays an essential role in Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) -associated NF-kappaB activation through its involvement in IKK activation, which then leads to subsequent IkappaB degradation and NF-kappaB nuclear translocation. In the present studies, we demonstrate a novel pathway in which IRAK-1 present in the nucleus participates in NF-kappaB-dependent gene expression. Nuclear localization of IRAK-1 is increased on cellular stimulation with IL-1 and LPS, or CRM-1-dependent nuclear export blockade. Induction of IRAK-1 produces enhanced NF-kappaB transcriptional activity that precedes IkappaB-alpha degradation and nuclear translocation of NF-kappaB. IRAK-1 binds to the promoter of NF-kappaB-regulated gene, IkappaB-alpha, and enhances binding of the NF-kappaB p65 subunit to NF-kappaB responsive elements within the IkappaB-alpha promoter. IRAK-1 phosphorylates histone H3 in vitro and is required for IL-1-induced phosphorylation of histone H3 at serine 10 in vivo. These data indicate that both cytosolic and nuclear actions of IRAK-1 participate in the activation of NF-kappaB-dependent transcriptional events.