Ibuprofen Metabolite Profiling Using a Combination of SPE/column-trapping and HPLC-micro-coil NMR

Overview

Journal J Pharm Biomed Anal

Specialty Chemistry

Date 2008 Feb 16

PMID 18276099

Citations 10

Authors

Danijel Djukovic

Emmanuel Appiah-Amponsah

Narasimhamurthy Shanaiah

G A Nagana Gowda

Ian Henry

Mike Everly

Brian Tobias

Daniel Raftery

Affiliations

Soon will be listed here.

Abstract

Solid-phase extraction and column-trapping preconcentration are combined to enhance HPLC-nuclear magnetic resonance (HPLC-NMR) and applied to metabolite profiling in biological samples. Combining the two signal enhancement techniques improved the NMR signal substantially such that we were able to identify 2-hydroxyibuprofen, carboxyibuprofen, and unmetabolized ibuprofen molecules from a small urine sample after a therapeutic dose of ibuprofen. The hyphenated SPE/column-trapping method resulted in an excellent overall signal enhancement of up to 90-fold.

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