Rapid and Inexpensive Analysis of Genetic Variability in Arapaima Gigas by PCR Multiplex Panel of Eight Microsatellites

Overview

Journal Genet Mol Res

Date 2008 Feb 15

PMID 18273816

Citations 5

Authors

I G Hamoy

E J M Santos

S E B Santos

Affiliations

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Abstract

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.

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