Solid-phase Capture of Pathogenic Bacteria by Using Gangliosides and Detection with Real-time PCR

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2008 Feb 12

PMID 18263751

Citations 8

Authors

Prerak T Desai

Marie K Walsh

Bart C Weimer

Affiliations

Soon will be listed here.

Abstract

We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of approximately 4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

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