Distribution of Atrial Natriuretic Peptide in the Conduction System and Ventricular Muscles of the Human Heart

Overview

Journal Virchows Arch A Pathol Anat Histopathol

Specialty Anatomy & Histology

Date 1991 Jan 1

PMID 1824903

Citations 5

Authors

N Mochizuki

H Sawa

H Yasuda

T Shinohara

K Nagashima

T Yamaji

N Ohnuma

W W Hall

Affiliations

Soon will be listed here.

Abstract

Atrial natriuretic peptide (ANP), a cardiac hormone, is known to be located in the atrial specific granules, but its presence and localization in the ventricular muscle of the human heart has not been examined fully. Using a specific antibody to human ANP, we studied the conduction system and ventricular muscle with immunohistochemical and ultrastructural methods in 30 hearts obtained at autopsy. These included 12 normal and 18 diseased hearts. In the normal hearts, ANP-positive granules, which were regularly observed in the atrial myocytes, were found in small quantities in the cells of the penetrating and branching bundles in 4 of 12, and in the cells of the ventricular free walls in 2 of the 12 hearts. In the diseased hearts, the positivity increased significantly (P less than 0.05), being found in 13 of 18 (72.2%) conduction systems and 10 of 18 (55.6%) ventricular muscles. The granules were confirmed to be immunoreactive with ANP by ultrastructural examination. Furthermore, the presence of ANP mRNA in the conduction system as well as in the ventricular myocytes was demonstrated by Northern blot hybridization for which we used the complementary DNA of human ANP. Thus, a small quantity of ANP appears to be synthesized and stored in the conduction system and ventricles of some normal hearts. However, ANP was shown to be present in a larger percentage of the diseased hearts.

Citing Articles

Natriuretic peptide immunoreactivity in nerve structures and Purkinje fibres of human, pig and sheep hearts.

Hansson M, Eriksson A, Forsgren S Histochem J. 1997; 29(4):329-36.

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Expression of renin and angiotensin-converting enzyme in human hearts.

Mochizuki N, Sawa H, Takada A, Okamoto H, Kawaguchi H, Nagashima K Heart Vessels. 1995; 10(6):285-93.

PMID: 8655465 DOI: 10.1007/BF02911386.

Presence of immunoreactive atrial natriuretic peptide in nerve fibres and conduction cells in the conduction system of the bovine heart.

Hansson M, Forsgren S Anat Embryol (Berl). 1993; 188(4):331-7.

PMID: 8279697 DOI: 10.1007/BF00185942.

Distribution of angiotensinogen in diseased human hearts.

Sawa H, Kawaguchi H, Mochizuki N, Endo Y, Kudo T, Tokuchi F Mol Cell Biochem. 1994; 132(1):15-23.

PMID: 8078504 DOI: 10.1007/BF00925670.

Immunoreactive atrial and brain natriuretic peptides are co-localized in Purkinje fibres but not in the innervation of the bovine heart conduction system.

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