The Photosystem II-associated Cah3 in Chlamydomonas Enhances the O2 Evolution Rate by Proton Removal

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2008 Feb 2

PMID 18239688

Citations 30

Authors

Tatiana Shutova

Hella Kenneweg

Joachim Buchta

Julia Nikitina

Vasily Terentyev

Sergey Chernyshov

Bertil Andersson

Suleyman I Allakhverdiev

Vyacheslav V Klimov

Holger Dau

Wolfgang Junge

Goran Samuelsson

Affiliations

Soon will be listed here.

Abstract

Water oxidation in photosystem II (PSII) is still insufficiently understood and is assumed to involve HCO(3)(-). A Chlamydomonas mutant lacking a carbonic anhydrase associated with the PSII donor side shows impaired O(2) evolution in the absence of HCO(3)(-). The O(2) evolution for saturating, continuous illumination (R(O2)) was slower than in the wild type, but was elevated by HCO(3)(-) and increased further by Cah3. The R(O2) limitation in the absence of Cah3/HCO(3)(-) was amplified by H(2)O/D(2)O exchange, but relieved by an amphiphilic proton carrier, suggesting a role of Cah3/HCO(3)(-) in proton translocation. Chlorophyll fluorescence indicates a Cah3/HCO(3)(-) effect at the donor side of PSII. Time-resolved delayed fluorescence and O(2)-release measurements suggest specific effects on proton-release steps but not on electron transfer. We propose that Cah3 promotes proton removal from the Mn complex by locally providing HCO(3)(-), which may function as proton carrier. Without Cah3, proton removal could become rate limiting during O(2) formation and thus, limit water oxidation under high light. Our results underlie the general importance of proton release at the donor side of PSII during water oxidation.

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