Secretion of Streptomyces Mobaraensis Pro-transglutaminase by Coryneform Bacteria

Overview

Journal Appl Microbiol Biotechnol

Specialties Biomedical Engineering
Biotechnology
Microbiology

Date 2008 Jan 26

PMID 18219481

Citations 9

Authors

Hiroshi Itaya

Yoshimi Kikuchi

Affiliations

Soon will be listed here.

Abstract

We previously reported on the secretion of Streptomyces mobaraensis transglutaminase by Corynebacterium glutamicum ATCC13869 (formerly classified as Brevibacterium lactofermentum). In the present work, we investigated whether any other coryneform bacteria showed higher productivity than C. glutamicum ATCC13869. We found that most coryneform species secreted pro-transglutaminase efficiently. Moreover, we confirmed that Corynebacterium ammoniagenes ATCC6872 produced about 2.5 g/l pro-transglutaminase over a 71-h period in a jar fermentor. Our findings suggest that some other coryneform bacteria, especially C. ammoniagenes ATCC6872, are potential hosts for industrial scale protein production.

Citing Articles

Efficient secretory production of proline/alanine/serine (PAS) biopolymers in Corynebacterium glutamicum yielding a monodisperse biological alternative to polyethylene glycol (PEG).

Friedrich L, Kikuchi Y, Matsuda Y, Binder U, Skerra A Microb Cell Fact. 2022; 21(1):227.

PMID: 36307781 PMC: 9616612. DOI: 10.1186/s12934-022-01948-5.

Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli under the control of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations.

Duarte L, Matte C, Cortivo P, Nunes J, Quadros Barse L, Bizarro C Braz J Microbiol. 2021; 52(3):1225-1233.

PMID: 34008152 PMC: 8324676. DOI: 10.1007/s42770-021-00521-3.

Production of transglutaminase in glutathione-producing recombinant Saccharomyces cerevisiae.

Hirono-Hara Y, Yui M, Hara K AMB Express. 2021; 11(1):13.

PMID: 33415535 PMC: 7790930. DOI: 10.1186/s13568-020-01176-3.

Transglutaminases: part I-origins, sources, and biotechnological characteristics.

Duarte L, Matte C, Bizarro C, Ayub M World J Microbiol Biotechnol. 2020; 36(1):15.

PMID: 31897837 DOI: 10.1007/s11274-019-2791-x.

Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis.

Hou Y, Chen S, Wang J, Liu G, Wu S, Tao Y BMC Biotechnol. 2019; 19(1):76.

PMID: 31718625 PMC: 6849255. DOI: 10.1186/s12896-019-0568-9.

References

Date M, Itaya H, Matsui H, Kikuchi Y . Secretion of human epidermal growth factor by Corynebacterium glutamicum. Lett Appl Microbiol. 2006; 42(1):66-70. DOI: 10.1111/j.1472-765X.2005.01802.x. View

Suzuki M, Taguchi S, Yamada S, Kojima S, MIURA K, Momose H . A novel member of the subtilisin-like protease family from Streptomyces albogriseolus. J Bacteriol. 1997; 179(2):430-8. PMC: 178713. DOI: 10.1128/jb.179.2.430-438.1997. View

Yokoyama K, Nio N, Kikuchi Y . Properties and applications of microbial transglutaminase. Appl Microbiol Biotechnol. 2004; 64(4):447-54. DOI: 10.1007/s00253-003-1539-5. View

Yokoyama K, Kunio O, Ohtsuka T, Nakamura N, Seguro K, Ejima D . In vitro refolding process of urea-denatured microbial transglutaminase without pro-peptide sequence. Protein Expr Purif. 2002; 26(2):329-35. DOI: 10.1016/s1046-5928(02)00536-3. View

Date M, Yokoyama K, Umezawa Y, Matsui H, Kikuchi Y . High level expression of Streptomyces mobaraensis transglutaminase in Corynebacterium glutamicum using a chimeric pro-region from Streptomyces cinnamoneus transglutaminase. J Biotechnol. 2004; 110(3):219-26. DOI: 10.1016/j.jbiotec.2004.02.011. View

Date M, Yokoyama K, Umezawa Y, Matsui H, Kikuchi Y . Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum. Appl Environ Microbiol. 2003; 69(5):3011-4. PMC: 154552. DOI: 10.1128/AEM.69.5.3011-3014.2003. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Kikuchi Y, Kojima H, Tanaka T, Takatsuka Y, Kamio Y . Characterization of a second lysine decarboxylase isolated from Escherichia coli. J Bacteriol. 1997; 179(14):4486-92. PMC: 179283. DOI: 10.1128/jb.179.14.4486-4492.1997. View

Kikuchi Y, Date M, Itaya H, Matsui K, Wu L . Functional analysis of the twin-arginine translocation pathway in Corynebacterium glutamicum ATCC 13869. Appl Environ Microbiol. 2006; 72(11):7183-92. PMC: 1636197. DOI: 10.1128/AEM.01528-06. View

10.

Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A . The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins. J Biotechnol. 2003; 104(1-3):5-25. DOI: 10.1016/s0168-1656(03)00154-8. View

11.

Kikuchi Y, Date M, Yokoyama K, Umezawa Y, Matsui H . Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-Like protease from Streptomyces albogriseolus. Appl Environ Microbiol. 2003; 69(1):358-66. PMC: 152470. DOI: 10.1128/AEM.69.1.358-366.2003. View

12.

Ikeda M, Nakagawa S . The Corynebacterium glutamicum genome: features and impacts on biotechnological processes. Appl Microbiol Biotechnol. 2003; 62(2-3):99-109. DOI: 10.1007/s00253-003-1328-1. View

13.

Choi J, Lee S . Secretory and extracellular production of recombinant proteins using Escherichia coli. Appl Microbiol Biotechnol. 2004; 64(5):625-35. DOI: 10.1007/s00253-004-1559-9. View

14.

Salim K, Haedens V, Content J, Leblon G, Huygen K . Heterologous expression of the Mycobacterium tuberculosis gene encoding antigen 85A in Corynebacterium glutamicum. Appl Environ Microbiol. 1997; 63(11):4392-400. PMC: 168759. DOI: 10.1128/aem.63.11.4392-4400.1997. View

15.

Hermann T . Industrial production of amino acids by coryneform bacteria. J Biotechnol. 2003; 104(1-3):155-72. DOI: 10.1016/s0168-1656(03)00149-4. View

16.

Kikuchi Y, Itaya H, Date M, Matsui K, Wu L . Production of Chryseobacterium proteolyticum protein-glutaminase using the twin-arginine translocation pathway in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2007; 78(1):67-74. DOI: 10.1007/s00253-007-1283-3. View

17.

Billman-Jacobe H, Wang L, Kortt A, Stewart D, Radford A . Expression and secretion of heterologous proteases by Corynebacterium glutamicum. Appl Environ Microbiol. 1995; 61(4):1610-3. PMC: 167416. DOI: 10.1128/aem.61.4.1610-1613.1995. View

18.

Liebl W, Sinskey A, Schleifer K . Expression, secretion, and processing of staphylococcal nuclease by Corynebacterium glutamicum. J Bacteriol. 1992; 174(6):1854-61. PMC: 205788. DOI: 10.1128/jb.174.6.1854-1861.1992. View