Electron Cryomicroscopy Reveals Different F1+F2 Protein States in Intact Parainfluenza Virions

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2008 Jan 25

PMID 18216117

Citations 24

Authors

Kai Ludwig

Boris Schade

Christoph Bottcher

Thomas Korte

Nina Ohlwein

Bolormaa Baljinnyam

Michael Veit

Andreas Herrmann

Affiliations

Soon will be listed here.

Abstract

Electron cryomicrographs of intact parainfluenza virus 5 (PIV5) virions revealed two different surface structures, namely, a continuous layer and distinct individual spikes. The structure of these spikes reconstructed from intact virions was compared with known F ectodomain structures and was found to be different from the prefusion PIV5 F0 structure but, surprisingly, very similar to the human PIV3 F postfusion structure. Hence, we conclude that the individual F1+F2 spikes in intact PIV5 virions also correspond to the postfusion state. Since the observed fusion activity of PIV5 virions has to be associated with prefusion F1+F2 proteins, they have necessarily to be localized in the continuous surface structure. The data therefore strongly suggest that the prefusion state of the F1+F2 protein requires stabilization, most probably by the association with hemagglutinin-neuraminidase. The conversion of F1+F2 proteins from the prefusion toward the postfusion state while embedded in the virus membrane is topologically difficult to comprehend on the basis of established models and demands reconsideration of our current understanding.

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