Detection of Viable but Nonculturable Escherichia Coli O157:H7 Bacteria in Drinking Water and River Water

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2008 Jan 22

PMID 18203853

Citations 24

Authors

Yanming Liu

Ainslie Gilchrist

Jing Zhang

Xing-Fang Li

Affiliations

Soon will be listed here.

Abstract

A sensitive method for specific detection of viable Escherichia coli O157:H7 cells, including viable but nonculturable (VBNC) cells, in water samples was developed. This method involved capture of the bacterial cells on a low-protein-binding membrane and direct extraction and purification of RNA followed by reverse transcription-PCR and electronic microarray detection of the rfbE and fliC genes of E. coli O157:H7. It detected as few as 1 CFU of E. coli O157:H7 in diluted cultures, 3 to 4 CFU/liter in tap water, 7 CFU/liter in river water, and 50 VBNC cells in 1 liter of river water, demonstrating the best limit of detection reported to date for VBNC cells in environmental water samples.

Citing Articles

Significance of Viable But Non-culturable (VBNC) State in Vibrios and Other Pathogenic Bacteria: Induction, Detection and the Role of Resuscitation Promoting Factors (Rpf).

Balagurusamy R, Gopi L, Kumar D, Viswanathan K, Meganathan V, Sathiyamurthy K Curr Microbiol. 2024; 81(12):417.

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A Photonic Immunosensor Detection Method for Viable and Non-Viable in Water Samples.

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Preliminary Study on Rapid and Simultaneous Detection of Viable O157:H7, s, and by PMA-mPCR in Food.

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Overview of VBNC, a survival strategy for microorganisms.

Izgordu O, Darcan C, Kariptas E 3 Biotech. 2022; 12(11):307.

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