Heterogeneous Nuclear Ribonucleoprotein D/AUF1 Interacts with Heterogeneous Nuclear Ribonucleoprotein L

Overview

Journal J Biosci

Specialties Biochemistry
Biology

Date 2008 Jan 19

PMID 18202450

Citations 5

Authors

Hong-Gyu Park

Ji-Young Yoon

Mieyoung Choi

Affiliations

Soon will be listed here.

Abstract

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is one of the principal pre-mRNA-binding proteins found in human cells. The hnRNP L protein is fairly abundant. However,it is not restricted to the nucleus, and instead shuttles between the nucleus and the cytoplasm. It is composed of 558 amino acid residues and harbours four loosely conserved RNP-consensus RNA-binding domains. In an attempt to characterize the interaction occurring between cellular proteins and hnRNP L, yeast two-hybrid screening was conducted using a HeLa cDNA library. Some of the cDNA clones were found to harbour a partial human hnRNP D/AUF1 cDNA (GeneBank accession number NM_031369). In this study,we determined that hnRNP L interacts specifically with the hnRNP D/AUF1 in the yeast two-hybrid system. This interaction was verified via an in vitro pull-down assay.

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References

Pinol-Roma S, Swanson M, Gall J, Dreyfuss G . A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts. J Cell Biol. 1989; 109(6 Pt 1):2575-87. PMC: 2115934. DOI: 10.1083/jcb.109.6.2575. View

Hahm B, Kim Y, Kim J, Kim T, Jang S . Heterogeneous nuclear ribonucleoprotein L interacts with the 3' border of the internal ribosomal entry site of hepatitis C virus. J Virol. 1998; 72(11):8782-8. PMC: 110294. DOI: 10.1128/JVI.72.11.8782-8788.1998. View

Hui J, Stangl K, Lane W, Bindereif A . HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat Struct Biol. 2002; 10(1):33-7. DOI: 10.1038/nsb875. View

Fawal M, Armstrong F, Ollier S, Dupont H, Touriol C, Monsarrat B . A "liaison dangereuse" between AUF1/hnRNPD and the oncogenic tyrosine kinase NPM-ALK. Blood. 2006; 108(8):2780-8. DOI: 10.1182/blood-2006-04-014902. View

Guang S, Felthauser A, Mertz J . Binding of hnRNP L to the pre-mRNA processing enhancer of the herpes simplex virus thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs. Mol Cell Biol. 2005; 25(15):6303-13. PMC: 1190326. DOI: 10.1128/MCB.25.15.6303-6313.2005. View

Kiledjian M, DeMaria C, Brewer G, Novick K . Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex. Mol Cell Biol. 1997; 17(8):4870-6. PMC: 232339. DOI: 10.1128/MCB.17.8.4870. View

Choi Y, Dreyfuss G . Isolation of the heterogeneous nuclear RNA-ribonucleoprotein complex (hnRNP): a unique supramolecular assembly. Proc Natl Acad Sci U S A. 1984; 81(23):7471-5. PMC: 392168. DOI: 10.1073/pnas.81.23.7471. View

Hui J, Hung L, Heiner M, Schreiner S, Neumuller N, Reither G . Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 2005; 24(11):1988-98. PMC: 1142610. DOI: 10.1038/sj.emboj.7600677. View

Feilotter H, Hannon G, Ruddell C, Beach D . Construction of an improved host strain for two hybrid screening. Nucleic Acids Res. 1994; 22(8):1502-3. PMC: 308013. DOI: 10.1093/nar/22.8.1502. View

10.

Kim J, Hahm B, Kim Y, Choi M, Jang S . Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. J Mol Biol. 2000; 298(3):395-405. DOI: 10.1006/jmbi.2000.3687. View

11.

Ishikawa F, Matunis M, Dreyfuss G, Cech T . Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 1993; 13(7):4301-10. PMC: 359985. DOI: 10.1128/mcb.13.7.4301-4310.1993. View

12.

Tong A, Nguyen J, Lynch K . Differential expression of CD45 isoforms is controlled by the combined activity of basal and inducible splicing-regulatory elements in each of the variable exons. J Biol Chem. 2005; 280(46):38297-304. DOI: 10.1074/jbc.M508123200. View

13.

Wagner B, DeMaria C, Sun Y, WILSON G, Brewer G . Structure and genomic organization of the human AUF1 gene: alternative pre-mRNA splicing generates four protein isoforms. Genomics. 1998; 48(2):195-202. DOI: 10.1006/geno.1997.5142. View

14.

Rothrock C, House A, Lynch K . HnRNP L represses exon splicing via a regulated exonic splicing silencer. EMBO J. 2005; 24(15):2792-802. PMC: 1182240. DOI: 10.1038/sj.emboj.7600745. View

15.

Melton A, Jackson J, Wang J, Lynch K . Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 2007; 27(19):6972-84. PMC: 2099238. DOI: 10.1128/MCB.00419-07. View

16.

Griffith B, Walsh C, Szeszel-Fedorowicz W, Timperman A, Salati L . Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing. Biochim Biophys Acta. 2006; 1759(11-12):552-61. PMC: 1828878. DOI: 10.1016/j.bbaexp.2006.10.001. View

17.

Krecic A, Swanson M . hnRNP complexes: composition, structure, and function. Curr Opin Cell Biol. 1999; 11(3):363-71. DOI: 10.1016/S0955-0674(99)80051-9. View

18.

Hahm B, Cho O, Kim J, Kim Y, Kim J, Oh Y . Polypyrimidine tract-binding protein interacts with HnRNP L. FEBS Lett. 1998; 425(3):401-6. DOI: 10.1016/s0014-5793(98)00269-5. View

19.

Tolnay M, Lambris J, Tsokos G . Transcriptional regulation of the complement receptor 2 gene: role of a heterogeneous nuclear ribonucleoprotein. J Immunol. 1998; 159(11):5492-501. View

20.

Dreyfuss G, Matunis M, Pinol-Roma S, Burd C . hnRNP proteins and the biogenesis of mRNA. Annu Rev Biochem. 1993; 62:289-321. DOI: 10.1146/annurev.bi.62.070193.001445. View