A Small Protein from the Bop-brp Intergenic Region of Halobacterium Salinarum Contains a Zinc Finger Motif and Regulates Bop and CrtB1 Transcription

Overview

Journal Mol Microbiol

Specialties Microbiology
Molecular Biology

Date 2008 Jan 9

PMID 18179416

Citations 23

Authors

Valery Y Tarasov

Huseyin Besir

Rita Schwaiger

Kathrin Klee

Katarina Furtwangler

Friedhelm Pfeiffer

Dieter Oesterhelt

Affiliations

Soon will be listed here.

Abstract

Bacteriorhodopsin, the photosynthetic protein of Halobacterium salinarum, is optimally expressed under anaerobic growth conditions. We identified Brz (OE3104F, bacteriorhodopsin-regulating zinc finger protein), a new regulator of the bop gene. It is a small protein with a zinc finger motif, encoded directly upstream of the bop gene in the same orientation. Deletion of the brz gene caused a large decrease of bop mRNA levels as shown by Northern blot and microarray analysis. A similar effect was obtained by site-directed mutagenesis of Cys and His residues in the zinc finger motif, indicating the importance of this motif for the function of the protein. In silico analysis of the genomes from H. salinarum and other archaea revealed a large family of similar small zinc finger motif proteins, some of which may also be involved in transcription regulation of their adjacent genes.

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