Genetic Determinants of Sindbis Virus Mosquito Infection Are Associated with a Highly Conserved Alphavirus and Flavivirus Envelope Sequence

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2007 Dec 28

PMID 18160430

Citations 13

Authors

Dennis J Pierro

Erik L Powers

Ken E Olson

Affiliations

Soon will be listed here.

Abstract

Wild-type Sindbis virus (SINV) strain MRE16 efficiently infects Aedes aegypti midgut epithelial cells (MEC), but laboratory-derived neurovirulent SINV strain TE/5'2J infects MEC poorly. SINV determinants for MEC infection have been localized to the E2 glycoprotein. The E2 amino acid sequences of MRE16 and TE/5'2J differ at 60 residue sites. To identify the genetic determinants of MEC infection of MRE16, the TE/5'2J virus genome was altered to contain either domain chimeras or more focused nucleotide substitutions of MRE16. The growth patterns of derived viruses in cell culture were determined, as were the midgut infection rates (MIR) in A. aegypti mosquitoes. The results showed that substitutions of MRE16 E2 aa 95 to 96 and 116 to 119 into the TE/5'2J virus increased MIR both independently and in combination with each other. In addition, a unique PPF/.GDS amino acid motif was located between these two sites that was found to be a highly conserved sequence among alphaviruses and flaviviruses but not other arboviruses.

Citing Articles

Prophylactic strategies to control chikungunya virus infection.

Hucke F, Bestehorn-Willmann M, Bugert J Virus Genes. 2021; 57(2):133-150.

PMID: 33590406 PMC: 7883954. DOI: 10.1007/s11262-020-01820-x.

Complexity of virus-vector interactions.

Kramer L Curr Opin Virol. 2016; 21:81-86.

PMID: 27580489 PMC: 5138088. DOI: 10.1016/j.coviro.2016.08.008.

Tissue Barriers to Arbovirus Infection in Mosquitoes.

Franz A, Kantor A, Passarelli A, Clem R Viruses. 2015; 7(7):3741-67.

PMID: 26184281 PMC: 4517124. DOI: 10.3390/v7072795.

Exposure of epitope residues on the outer face of the chikungunya virus envelope trimer determines antibody neutralizing efficacy.

Fong R, Banik S, Mattia K, Barnes T, Tucker D, Liss N J Virol. 2014; 88(24):14364-79.

PMID: 25275138 PMC: 4249124. DOI: 10.1128/JVI.01943-14.

Subgenomic reporter RNA system for detection of alphavirus infection in mosquitoes.

Steel J, Franz A, Sanchez-Vargas I, Olson K, Geiss B PLoS One. 2013; 8(12):e84930.

PMID: 24367703 PMC: 3868651. DOI: 10.1371/journal.pone.0084930.

References

Klimstra W, Ryman K, Bernard K, Nguyen K, Biron C, Johnston R . Infection of neonatal mice with sindbis virus results in a systemic inflammatory response syndrome. J Virol. 1999; 73(12):10387-98. PMC: 113094. DOI: 10.1128/JVI.73.12.10387-10398.1999. View

Rost B, Sander C . Improved prediction of protein secondary structure by use of sequence profiles and neural networks. Proc Natl Acad Sci U S A. 1993; 90(16):7558-62. PMC: 47181. DOI: 10.1073/pnas.90.16.7558. View

Delos S, Gilbert J, White J . The central proline of an internal viral fusion peptide serves two important roles. J Virol. 2000; 74(4):1686-93. PMC: 111643. DOI: 10.1128/jvi.74.4.1686-1693.2000. View

Olson K, Myles K, Seabaugh R, Higgs S, Carlson J, Beaty B . Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection. Insect Mol Biol. 2000; 9(1):57-65. DOI: 10.1046/j.1365-2583.2000.00162.x. View

Lee E, Lobigs M . Substitutions at the putative receptor-binding site of an encephalitic flavivirus alter virulence and host cell tropism and reveal a role for glycosaminoglycans in entry. J Virol. 2000; 74(19):8867-75. PMC: 102081. DOI: 10.1128/jvi.74.19.8867-8875.2000. View

Bernard K, Klimstra W, Johnston R . Mutations in the E2 glycoprotein of Venezuelan equine encephalitis virus confer heparan sulfate interaction, low morbidity, and rapid clearance from blood of mice. Virology. 2000; 276(1):93-103. DOI: 10.1006/viro.2000.0546. View

Tan B, Nason E, Staeuber N, Jiang W, Monastryrskaya K, Roy P . RGD tripeptide of bluetongue virus VP7 protein is responsible for core attachment to Culicoides cells. J Virol. 2001; 75(8):3937-47. PMC: 114884. DOI: 10.1128/JVI.75.8.3937-3947.2001. View

Hurrelbrink R, McMinn P . Attenuation of Murray Valley encephalitis virus by site-directed mutagenesis of the hinge and putative receptor-binding regions of the envelope protein. J Virol. 2001; 75(16):7692-702. PMC: 115004. DOI: 10.1128/JVI.75.16.7692-7702.2001. View

Chambers T, Nickells M . Neuroadapted yellow fever virus 17D: genetic and biological characterization of a highly mouse-neurovirulent virus and its infectious molecular clone. J Virol. 2001; 75(22):10912-22. PMC: 114671. DOI: 10.1128/JVI.75.22.10912-10922.2001. View

10.

Serafin I, Aaskov J . Identification of epitopes on the envelope (E) protein of dengue 2 and dengue 3 viruses using monoclonal antibodies. Arch Virol. 2002; 146(12):2469-79. DOI: 10.1007/s007050170017. View

11.

Zhang W, Mukhopadhyay S, Pletnev S, Baker T, Kuhn R, Rossmann M . Placement of the structural proteins in Sindbis virus. J Virol. 2002; 76(22):11645-58. PMC: 136788. DOI: 10.1128/jvi.76.22.11645-11658.2002. View

12.

Tugizov S, Berline J, Palefsky J . Epstein-Barr virus infection of polarized tongue and nasopharyngeal epithelial cells. Nat Med. 2003; 9(3):307-14. DOI: 10.1038/nm830. View

13.

Pierro D, Myles K, Foy B, Beaty B, Olson K . Development of an orally infectious Sindbis virus transducing system that efficiently disseminates and expresses green fluorescent protein in Aedes aegypti. Insect Mol Biol. 2003; 12(2):107-16. DOI: 10.1046/j.1365-2583.2003.00392.x. View

14.

Saleh S, Poidinger M, Mackenzie J, Broom A, Lindsay M, Hall R . Complete genomic sequence of the Australian south-west genotype of Sindbis virus: comparisons with other Sindbis strains and identification of a unique deletion in the 3'-untranslated region. Virus Genes. 2003; 26(3):317-27. DOI: 10.1023/a:1024407516352. View

15.

Myles K, Pierro D, Olson K . Deletions in the putative cell receptor-binding domain of Sindbis virus strain MRE16 E2 glycoprotein reduce midgut infectivity in Aedes aegypti. J Virol. 2003; 77(16):8872-81. PMC: 167217. DOI: 10.1128/jvi.77.16.8872-8881.2003. View

16.

Nickells M, Chambers T . Neuroadapted yellow fever virus 17D: determinants in the envelope protein govern neuroinvasiveness for SCID mice. J Virol. 2003; 77(22):12232-42. PMC: 254278. DOI: 10.1128/jvi.77.22.12232-12242.2003. View

17.

Hung J, Hsieh M, Young M, Kao C, King C, Chang W . An external loop region of domain III of dengue virus type 2 envelope protein is involved in serotype-specific binding to mosquito but not mammalian cells. J Virol. 2003; 78(1):378-88. PMC: 303388. DOI: 10.1128/jvi.78.1.378-388.2004. View

18.

Myles K, Pierro D, Olson K . Comparison of the transmission potential of two genetically distinct Sindbis viruses after oral infection of Aedes aegypti (Diptera: Culicidae). J Med Entomol. 2004; 41(1):95-106. DOI: 10.1603/0022-2585-41.1.95. View

19.

Roehrig J, Risi P, Brubaker J, HUNT A, Beaty B, TRENT D . T-helper cell epitopes on the E-glycoprotein of dengue 2 Jamaica virus. Virology. 1994; 198(1):31-8. DOI: 10.1006/viro.1994.1005. View

20.

Rost B, Sander C . Conservation and prediction of solvent accessibility in protein families. Proteins. 1994; 20(3):216-26. DOI: 10.1002/prot.340200303. View