Induction of CYP1A2 by Heavy Coffee Consumption in Serbs and Swedes

Overview

Journal Eur J Clin Pharmacol

Specialty Pharmacology

Date 2007 Dec 25

PMID 18157525

Citations 33

Authors

Natasa Djordjevic

Roza Ghotbi

Leif Bertilsson

Slobodan Jankovic

Eleni Aklillu

Affiliations

Soon will be listed here.

Abstract

Objectives: To investigate the influence of coffee consumption on CYP1A2 enzyme activity controlling for the effects of smoking and oral contraceptive (OC) use among Serbs and Swedes and to compare CYP1A2 activity between the two populations.

Methods: Data on oral contraceptive use, habitual coffee consumption and smoking habits were obtained from 100 Serbian and 149 Swedish healthy volunteers using a detailed questionnaire. CYP1A2 activity was estimated by plasma paraxanthine/caffeine (17X/137X) ratio analysed by reversed-phase HPLC after oral administration of 100 mg caffeine.

Results: Daily consumption of at least three cups of coffee significantly increased CYP1A2 enzyme activity in both Serbs (P=0.0002) and Swedes (P<0.0001). Among non-smokers and non-OC users, heavy coffee consumption significantly increased CYP1A2 activity in Serbs (mean difference 0.11; 95% CI of the mean difference 0.04, 0.18; P=0.003) and Swedes (mean difference 0.07; 95% CI of the mean difference 0.01, 0.12; P=0.02). Significantly higher 17X/137X ratio was detected in Serbian smokers compared to non-smokers. There was no significant gender difference in CYP1A2 activity in Serbs. Controlling for the effect of smoking, heavy coffee consumption habit and oral contraceptive use, significantly lower 17X/137X ratio was observed in Serbs than in Swedes (P=0.0003).

Conclusions: Habitual heavy coffee consumption increases CYP1A2 activity. Polycyclic aromatic hydrocarbons formed during roasting of coffee beans might partly be responsible for this effect. The reason for the observed lower CYP1A2 activity in Serbs as compared to Swedes remains to be investigated.

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References

Horn E, Tucker M, Lambert G, Silverman D, Zametkin D, Sinha R . A study of gender-based cytochrome P4501A2 variability: a possible mechanism for the male excess of bladder cancer. Cancer Epidemiol Biomarkers Prev. 1995; 4(5):529-33. View

Ghotbi R, Christensen M, Roh H, Ingelman-Sundberg M, Aklillu E, Bertilsson L . Comparisons of CYP1A2 genetic polymorphisms, enzyme activity and the genotype-phenotype relationship in Swedes and Koreans. Eur J Clin Pharmacol. 2007; 63(6):537-46. DOI: 10.1007/s00228-007-0288-2. View

Relling M, Lin J, Ayers G, Evans W . Racial and gender differences in N-acetyltransferase, xanthine oxidase, and CYP1A2 activities. Clin Pharmacol Ther. 1992; 52(6):643-58. DOI: 10.1038/clpt.1992.203. View

. Coffee, tea, mate, methylxanthines and methylglyoxal. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Lyon, 27 February to 6 March 1990. IARC Monogr Eval Carcinog Risks Hum. 1991; 51:1-513. PMC: 7681554. View

Chen M . Ethnic or racial differences revisited: impact of dosage regimen and dosage form on pharmacokinetics and pharmacodynamics. Clin Pharmacokinet. 2006; 45(10):957-64. DOI: 10.2165/00003088-200645100-00001. View

Aklillu E, Carrillo J, Makonnen E, Hellman K, Pitarque M, Bertilsson L . Genetic polymorphism of CYP1A2 in Ethiopians affecting induction and expression: characterization of novel haplotypes with single-nucleotide polymorphisms in intron 1. Mol Pharmacol. 2003; 64(3):659-69. DOI: 10.1124/mol.64.3.659. View

Okey A . Enzyme induction in the cytochrome P-450 system. Pharmacol Ther. 1990; 45(2):241-98. DOI: 10.1016/0163-7258(90)90030-6. View

Han X, Ouyang D, Chen X, Shu Y, Jiang C, Tan Z . Inducibility of CYP1A2 by omeprazole in vivo related to the genetic polymorphism of CYP1A2. Br J Clin Pharmacol. 2002; 54(5):540-3. PMC: 1874453. DOI: 10.1046/j.1365-2125.2002.01686.x. View

Rasmussen B, Maenpaa J, Pelkonen O, Loft S, Poulsen H, Lykkesfeldt J . Selective serotonin reuptake inhibitors and theophylline metabolism in human liver microsomes: potent inhibition by fluvoxamine. Br J Clin Pharmacol. 1995; 39(2):151-9. PMC: 1364952. DOI: 10.1111/j.1365-2125.1995.tb04422.x. View

10.

Ayalogu E, Snelling J, Lewis D, Talwar S, Clifford M, Ioannides C . Induction of hepatic CYP1A2 by the oral administration of caffeine to rats: lack of association with the Ah locus. Biochim Biophys Acta. 1995; 1272(2):89-94. DOI: 10.1016/0925-4439(95)00071-b. View

11.

Tantcheva-Poor I, Zaigler M, Rietbrock S, Fuhr U . Estimation of cytochrome P-450 CYP1A2 activity in 863 healthy Caucasians using a saliva-based caffeine test. Pharmacogenetics. 1999; 9(2):131-44. View

12.

Parker A, Pritchard P, Preston T, Choonara I . Induction of CYP1A2 activity by carbamazepine in children using the caffeine breath test. Br J Clin Pharmacol. 1998; 45(2):176-8. PMC: 1873361. DOI: 10.1046/j.1365-2125.1998.00684.x. View

13.

Houessou J, Benac C, Delteil C, Camel V . Determination of polycyclic aromatic hydrocarbons in coffee brew using solid-phase extraction. J Agric Food Chem. 2005; 53(4):871-9. DOI: 10.1021/jf048633a. View

14.

Fontana R, Lown K, Paine M, Fortlage L, Santella R, FELTON J . Effects of a chargrilled meat diet on expression of CYP3A, CYP1A, and P-glycoprotein levels in healthy volunteers. Gastroenterology. 1999; 117(1):89-98. DOI: 10.1016/s0016-5085(99)70554-8. View

15.

Carrillo J, Christensen M, Ramos S, Alm C, Dahl M, Benitez J . Evaluation of caffeine as an in vivo probe for CYP1A2 using measurements in plasma, saliva, and urine. Ther Drug Monit. 2000; 22(4):409-17. DOI: 10.1097/00007691-200008000-00008. View

16.

KALOW W, Tang B . Use of caffeine metabolite ratios to explore CYP1A2 and xanthine oxidase activities. Clin Pharmacol Ther. 1991; 50(5 Pt 1):508-19. DOI: 10.1038/clpt.1991.176. View

17.

Ma X, Idle J, Krausz K, Gonzalez F . Metabolism of melatonin by human cytochromes p450. Drug Metab Dispos. 2004; 33(4):489-94. DOI: 10.1124/dmd.104.002410. View

18.

Masimirembwa C, Beke M, Hasler J, Tang B, KALOW W . Low CYP1A2 activity in rural Shona children of Zimbabwe. Clin Pharmacol Ther. 1995; 57(1):25-31. DOI: 10.1016/0009-9236(95)90262-7. View

19.

Zhou H, Josephy P, Kim D, Guengerich F . Functional characterization of four allelic variants of human cytochrome P450 1A2. Arch Biochem Biophys. 2004; 422(1):23-30. DOI: 10.1016/j.abb.2003.11.019. View

20.

Birkett D, McManus M, Tassaneeyakul W, Veronese M, Andersson T, Tukey R . Caffeine metabolism by human hepatic cytochromes P450: contributions of 1A2, 2E1 and 3A isoforms. Biochem Pharmacol. 1994; 47(10):1767-76. DOI: 10.1016/0006-2952(94)90304-2. View