Suppression of Polarity of Insertion Mutations in the Gal Operon and N Mutations in Bacteriophage Lambda

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1976 Jun 1

PMID 181361

Citations 14

Authors

O Reyes

M Gottesman

S Adhya

Affiliations

Soon will be listed here.

Abstract

Bacterial mutations (psuA and psu) known for their ability to suppress the polarity on nonsense mutations are shown to suppress the polarity of certain insertion mutations in the gal operon. The short insertion, IS1 (800 nucleotide pairs), is about 15 to 50% suppressed, whereas longer insertions, IS2 (1,400 nucleotide pairs), and IS3 (1,200 nucleotide pairs), are not. Some of the polarity suppressor mutations (psu-1, psu-2, and psu-3) are at least partially permissive for N-gene mutations (N7 and N53) of bacteriophage lambda, suggesting a relationship between natural and mutational polar signals. That this relationship may be complex is indicated by the fact that other suppressor mutations, effective in suppressing nonsense or insertion polarity, fail entirely to permit the growth of lambda N mutants.

Citing Articles

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Suppression of polarity in the gal operon by ultraviolet irradiation.

Fassler J, Tessman I J Bacteriol. 1980; 144(3):1205-7.

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Relation between UV suppression of polarity in phi X174 and UV sensitivity of rho mutants.

Fassler J, Tessman I J Virol. 1981; 37(3):955-62.

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Autoregulation of the rho gene of Escherichia coli K-12.

Kung H, Bekesi E, Guterman S, Gray J, Traub L, Calhoun D Mol Gen Genet. 1984; 193(2):210-3.

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Antitermination of transcription from an Escherichia coli ribosomal RNA promoter.

Holben W, Morgan E Proc Natl Acad Sci U S A. 1984; 81(21):6789-93.

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