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Determination of Specific Growth Rate by Measurement of Specific Rate of Ribosome Synthesis in Growing and Nongrowing Cultures of Acinetobacter Calcoaceticus

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Date 2007 Dec 18
PMID 18083876
Citations 3
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Abstract

RT-RiboSyn measures the specific rate of ribosome synthesis in distinct microbial populations by measuring the generation rate of precursor 16S rRNA relative to that of mature 16S rRNA when precursor 16S rRNA processing is inhibited. Good agreement was demonstrated between specific rate of ribosome synthesis and specific growth rate of Acinetobacter calcoaceticus.

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References
1.
Stahl D, Flesher B, MANSFIELD H, Montgomery L . Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Appl Environ Microbiol. 1988; 54(5):1079-84. PMC: 202606. DOI: 10.1128/aem.54.5.1079-1084.1988. View

2.
Schmid M, Schmitz-Esser S, Jetten M, Wagner M . 16S-23S rDNA intergenic spacer and 23S rDNA of anaerobic ammonium-oxidizing bacteria: implications for phylogeny and in situ detection. Environ Microbiol. 2001; 3(7):450-9. DOI: 10.1046/j.1462-2920.2001.00211.x. View

3.
Stroot P, Oerther D . Elevated precursor 16S rRNA levels suggest the presence of growth inhibitors in wastewater. Water Sci Technol. 2003; 47(11):241-50. View

4.
Brock T . CHLORAMPHENICOL. Bacteriol Rev. 1961; 25(1):32-48. PMC: 441072. DOI: 10.1128/br.25.1.32-48.1961. View

5.
Oerther D, van Loosdrecht M, Raskin L . Quantifying the impact of wastewater micronutrient composition on in situ growth activity of Acinetobacter spp. Water Sci Technol. 2002; 46(1-2):443-7. View