» Articles » PMID: 18082466

Bone Morphogenetic Protein-7 Protects Human Intervertebral Disc Cells in Vitro from Apoptosis

Overview
Journal Spine J
Specialty Orthopedics
Date 2007 Dec 18
PMID 18082466
Citations 37
Authors
Affiliations
Soon will be listed here.
Abstract

Background Context: Disc degeneration includes dysfunction and loss of disc cells leading to a decrease in extracellular matrix (ECM) components. Apoptosis has been identified in degenerated discs. Bone morphogenetic protein-7 (BMP-7) has been reported to stimulate ECM synthesis in the intervertebral disc (IVD), but its effect on disc cell viability is unknown.

Purpose: To investigate whether BMP-7 can protect disc cells from programmed cell death while enhancing ECM production.

Study Design: An in vitro study to examine the effect of BMP-7 on apoptosis of IVD cells.

Methods: Human nucleus pulposus (NP) cells were cultured in monolayer, and human recombinant pure BMP-7 (rhBMP-7) was added to the medium when the cells were in the second passage. Thereafter, apoptosis was induced by either tumor necrosis factor-alpha (TNF-alpha) or hydrogen peroxide (H(2)O(2)). Cellular apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and caspase-3 activity. ECM synthesis was assessed by immunofluorescence for collagen-2 and aggrecan. To study the possibility of bone induction by rhBMP-7 in disc cells, alkaline phosphatase activity and Alizarin red-S staining were evaluated.

Results: Apoptosis was induced by both TNF-alpha and H(2)O(2). Addition of rhBMP-7 resulted in inhibition of the apoptotic effects caused by both inducers. Further, BMP-7 decreased caspase-3 activity. In the presence of BMP-7, ECM production was maintained by the cells despite being in an apoptotic environment. No osteoblastic induction of the disc cells was seen.

Conclusions: BMP-7 was demonstrated to prevent apoptosis of human disc cells in vitro. One of the antiapoptotic effects of BMP-7 on NP cells might be a result of its inactivation of caspase-3. Collagen production was maintained by addition of rhBMP-7 in an apoptotic environment.

Citing Articles

Custom-Made Ce-Mn Bimetallic Nanozyme for the Treatment of Intervertebral Disc Degeneration by Inhibiting Oxidative Stress and Modulating Macrophage M1/M2 Polarization.

Wu J, Chen Z, Huang H, Wang H, Wang X, Lu Z Biomater Res. 2024; 28:0118.

PMID: 39717477 PMC: 11665849. DOI: 10.34133/bmr.0118.


Bone morphogenetic proteins, DNA methylation, and gut microbiota interaction in lumbar disc degeneration: A multi-omics Mendelian randomization study.

Li X, Wang P, Wang Q, Wang D, Wang S, Wang Y JOR Spine. 2024; 7(4):e70027.

PMID: 39713086 PMC: 11659950. DOI: 10.1002/jsp2.70027.


Significance of Oxidative Stress in the Diagnosis and Subtype Classification of Intervertebral Disc Degeneration.

Gu J, Zhou X, Xie L Biochem Genet. 2023; 62(1):193-207.

PMID: 37314550 DOI: 10.1007/s10528-023-10412-x.


Molecular Basic of Pharmacotherapy of Cytokine Imbalance as a Component of Intervertebral Disc Degeneration Treatment.

Shnayder N, Ashkhotov A, Trefilova V, Nurgaliev Z, Novitsky M, Petrova M Int J Mol Sci. 2023; 24(9).

PMID: 37175399 PMC: 10178334. DOI: 10.3390/ijms24097692.


Mechanobiology of MicroRNAs in Intervertebral Disk Degeneration.

Supra R, Agrawal D J Spine Res Surg. 2023; 5(1):1-9.

PMID: 36777190 PMC: 9912327. DOI: 10.26502/fjsrs0051.