Deletion of the Core-H Region in Mice Abolishes the Expression of Three Proximal Odorant Receptor Genes in Cis

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2007 Dec 14

PMID 18077433

Citations 50

Authors

Hirofumi Nishizumi

Kouhei Kumasaka

Nobuko Inoue

Ai Nakashima

Hitoshi Sakano

Affiliations

Soon will be listed here.

Abstract

We have previously reported that a 2.1-kb homology (H) sequence, conserved between mouse and human, regulates the odorant receptor (OR) gene MOR28 in transgenic mice. Here, we narrowed down the essential sequences of the H to a core of 124 bp by using a transient expression system in zebrafish embryos. Transgenic experiments in mice demonstrated that the core-H sequence is sufficient to endow expression of the MOR28 minigene. Deletion and mutation analyses of the core-H region revealed two homeodomain sequences to be essential for the H enhancer activity. Targeted deletion of the core-H abolished expression of three proximal OR genes, MOR28, MOR10, and MOR83, in cis, indicating the presence of another locus control region/enhancer in the downstream region, that regulates four distal OR genes in the same MOR28 cluster. In the heterozygous mice, the H(-) phenotype of the mutant allele was not rescued by the wild-type H(+) allele in trans.

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