Protein Folding Following Synthesis in Vitro and in Vivo: Association of Newly Synthesized Protein with 50S Subunit of E. Coli Ribosome
Overview
Authors
Affiliations
In the accompanying paper, it was shown that a protein, while reverting to native form from the unfolded state in vitro with the help of bacterial 70S ribosome, split the latter into its subunits (50S and 30S) and remains associated with the 50S subunit. Here, we follow the fate of nascent proteins both in case of in vivo and in vitro translation system. The newly synthesised protein was found to associate with the 50S subunit in both the cases.
Roles of Nucleic Acids in Protein Folding, Aggregation, and Disease.
Litberg T, Horowitz S ACS Chem Biol. 2024; 19(4):809-823.
PMID: 38477936 PMC: 11149768. DOI: 10.1021/acschembio.3c00695.
Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex.
Tuttle L, Pacheco D, Warfield L, Luo J, Ranish J, Hahn S Cell Rep. 2018; 22(12):3251-3264.
PMID: 29562181 PMC: 5908246. DOI: 10.1016/j.celrep.2018.02.097.
The double life of the ribosome: When its protein folding activity supports prion propagation.
Voisset C, Blondel M, Jones G, Friocourt G, Stahl G, Chedin S Prion. 2017; 11(2):89-97.
PMID: 28362551 PMC: 5399909. DOI: 10.1080/19336896.2017.1303587.
A Possible Role of the Full-Length Nascent Protein in Post-Translational Ribosome Recycling.
Das D, Samanta D, Bhattacharya A, Basu A, Das A, Ghosh J PLoS One. 2017; 12(1):e0170333.
PMID: 28099529 PMC: 5242463. DOI: 10.1371/journal.pone.0170333.
Protein Folding Activity of the Ribosome is involved in Yeast Prion Propagation.
Blondel M, Soubigou F, Evrard J, Nguyen P, Hasin N, Chedin S Sci Rep. 2016; 6():32117.
PMID: 27633137 PMC: 5025663. DOI: 10.1038/srep32117.