Developments in Directed Evolution for Improving Enzyme Functions

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2007 Dec 7

PMID 18057449

Citations 23

Authors

S Sen

V Venkata Dasu

B Mandal

Affiliations

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Abstract

The engineering of enzymes with altered activity, specificity, and stability, using directed evolution techniques that mimic evolution on a laboratory timescale, is now well established. In vitro recombination techniques such as DNA shuffling, staggered extension process (StEP), random chimeragenesis on transient templates (RACHITT), iterative truncation for the creation of hybrid enzymes (ITCHY), recombined extension on truncated templates (RETT), and so on have been developed to mimic and accelerate nature's recombination strategy. This review discusses gradual advances in the techniques and strategies used for the directed evolution of biocatalytic enzymes aimed at improving the quality and potential of enzyme libraries, their advantages, and disadvantages.

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