The Lengthening of a Giant Protein: When, How, and Why?

Overview

Journal J Mol Evol

Specialty Biochemistry

Date 2007 Nov 30

PMID 18046595

Citations 20

Authors

Olivier Meiniel

Robert Meiniel

Fabrice Lalloue

Robert Didier

Marie-Odile Jauberteau

Annie Meiniel

Daniel Petit

Affiliations

Soon will be listed here.

Abstract

Subcommissural organ (SCO)-spondin is a giant glycoprotein of more than 5000 amino acids found in Vertebrata, expressed in the central nervous system and constitutive of Reissner's fiber. For the first time, in situ hybridization performed on zebrafish (Danio rerio) embryos shows that the gene encoding this protein is expressed transitionally in the floor plate, the ventral midline of the neural tube, and later in the diencephalic third ventricle roof, the SCO. The modular organization of the protein in Echinodermata (Strongylocentrotus purpuratus), Urochordata (Ciona savignyi and C. intestinalis), and Vertebrata (Teleostei, Amphibia, Aves and Mammalia) is also described. As the thrombospondin type 1 repeat motifs represent an increasingly large part of the protein during Deuterostomia evolution, the duplication mechanisms leading to this complex organization are examined. The functional significance of the particularly well-preserved arrangement of the series of SCO-spondin repeat motifs and thombospondin type 1 repeats is discussed.

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