DNA Methyltransferase 3B Promoter Polymorphism and Its Susceptibility to Primary Hepatocellular Carcinoma in the Chinese Han Nationality Population: a Case-control Study

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2007 Nov 21

PMID 18023104

Citations 16

Authors

Ying Wu

Ju-Sheng Lin

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the correlation between C/T single nucleotide polymorphism (SNP) in the promoter of the DNA methyltransferase 3B (DNMT3B) gene and risk for development and progression of primary hepatocellular carcinoma (HCC).

Methods: One hundred case subjects were selected consecutively from Tongji Hospital (Wuhan, China). from March to November 2006. They did not receive radiotherapy or chemotherapy for newly diagnosed and histopathologically confirmed HCC. One hundred and forty control subjects having no history of cancerous or genetic diseases were healthy volunteers to Wuhan Blood Center in the same period. Frequency was matched for sex, age, alcohol consumption and cigarette smoking status of the case subjects. C/T polymorphism of the DNMT3B promoter was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing analysis. The association between genotypes of DNMT3B and clinicopathological parameters among cases was also studied.

Results: The CC genotype was not detected in both HCC patients and controls. In control subjects, the frequency of TT and CT genotypes was 99.3% and 0.7% respectively, and that of T and C alleles was 99.6% and 0.4% respectively. The frequency of CT genotype was higher in HCC (3.0%). The frequency of T and C alleles was 98.5% and 1.5% respectively. However, the genotype and allelotype distribution in HCC patients was not significantly different from that in controls.

Conclusion: C/T polymorphism is not associated with the increased risk of HCC. DNMT3B genetic polymorphism is variable in different races, ethnic groups or geographic areas. Further study is needed to clarify the role of DNMT3B SNP in the development of HCC among other populations.

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References

Robertson K . DNA methylation, methyltransferases, and cancer. Oncogene. 2001; 20(24):3139-55. DOI: 10.1038/sj.onc.1204341. View

Tang Z . Hepatocellular carcinoma--cause, treatment and metastasis. World J Gastroenterol. 2002; 7(4):445-54. PMC: 4688653. DOI: 10.3748/wjg.v7.i4.445. View

Wang Y, Wang R, Wen D, Li Y, Guo W, Wang N . Single nucleotide polymorphism in DNA methyltransferase 3B promoter and its association with gastric cardiac adenocarcinoma in North China. World J Gastroenterol. 2005; 11(23):3623-7. PMC: 4315975. DOI: 10.3748/wjg.v11.i23.3623. View

Shames D, Minna J, Gazdar A . DNA methylation in health, disease, and cancer. Curr Mol Med. 2007; 7(1):85-102. DOI: 10.2174/156652407779940413. View

Esteller M . Epigenetics provides a new generation of oncogenes and tumour-suppressor genes. Br J Cancer. 2007; 96 Suppl:R26-30. View

Park H, Yu E, Shim Y . DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma. Cancer Lett. 2005; 233(2):271-8. DOI: 10.1016/j.canlet.2005.03.017. View

Turek-Plewa J, Jagodzinski P . The role of mammalian DNA methyltransferases in the regulation of gene expression. Cell Mol Biol Lett. 2005; 10(4):631-47. View

Robertson K, Uzvolgyi E, Liang G, Talmadge C, Sumegi J, Gonzales F . The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors. Nucleic Acids Res. 1999; 27(11):2291-8. PMC: 148793. DOI: 10.1093/nar/27.11.2291. View

Wang L, Rodriguez M, Kim E, Xu Y, Bekele N, El-Naggar A . A novel C/T polymorphism in the core promoter of human de novo cytosine DNA methyltransferase 3B6 is associated with prognosis in head and neck cancer. Int J Oncol. 2004; 25(4):993-9. View

10.

Jin F, Dowdy S, Xiong Y, Eberhardt N, Podratz K, Jiang S . Up-regulation of DNA methyltransferase 3B expression in endometrial cancers. Gynecol Oncol. 2005; 96(2):531-8. DOI: 10.1016/j.ygyno.2004.10.039. View

11.

Oh B, Kim H, Park H, Shim Y, Choi J, Park C . DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation. Int J Mol Med. 2007; 20(1):65-73. View

12.

Vaniushin B . [DNA methylation and epigenetics]. Genetika. 2006; 42(9):1186-99. View

13.

Tannapfel A . [Methylation and other new concepts for the origin of hepatocellular carcinoma]. Pathologe. 2006; 27(4):284-8. DOI: 10.1007/s00292-006-0837-y. View

14.

Yoshikawa H . [DNA methylation and cancer]. Gan To Kagaku Ryoho. 2007; 34(2):145-9. View

15.

Lin R, Hsu H, Chang J, Chen C, Chen J, Wang Y . Alteration of DNA methyltransferases contributes to 5'CpG methylation and poor prognosis in lung cancer. Lung Cancer. 2006; 55(2):205-13. DOI: 10.1016/j.lungcan.2006.10.022. View

16.

Eng C, Herman J, Baylin S . A bird's eye view of global methylation. Nat Genet. 2000; 24(2):101-2. DOI: 10.1038/72730. View

17.

Szyf M . Targeting DNA methylation in cancer. Bull Cancer. 2006; 93(9):961-72. View

18.

Montgomery K, Liu M, Eccles D, Campbell I . The DNMT3B C-->T promoter polymorphism and risk of breast cancer in a British population: a case-control study. Breast Cancer Res. 2004; 6(4):R390-4. PMC: 468658. DOI: 10.1186/bcr807. View

19.

Aung P, Matsumura S, Kuraoka K, Kunimitsu K, Yoshida K, Matsusaki K . No evidence of correlation between the single nucleotide polymorphism of DNMT3B promoter and gastric cancer risk in a Japanese population. Oncol Rep. 2005; 14(5):1151-4. View

20.

Soejima H, Zhao W, Mukai T . Epigenetic silencing of the MGMT gene in cancer. Biochem Cell Biol. 2005; 83(4):429-37. DOI: 10.1139/o05-140. View