A Novel Caffeine Dehydrogenase in Pseudomonas Sp. Strain CBB1 Oxidizes Caffeine to Trimethyluric Acid

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2007 Nov 6

PMID 17981969

Citations 15

Authors

Chi Li Yu

Yogesh Kale

Sridhar Gopishetty

Tai Man Louie

Mani Subramanian

Affiliations

Soon will be listed here.

Abstract

A unique heterotrimeric caffeine dehydrogenase was purified from Pseudomonas sp. strain CBB1. This enzyme oxidized caffeine to trimethyluric acid stoichiometrically and hydrolytically, without producing hydrogen peroxide. The enzyme was not NAD(P)(+) dependent; coenzyme Q(0) was the preferred electron acceptor. The enzyme was specific for caffeine and theobromine and showed no activity with xanthine.

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