A Role of the Transient Receptor Potential Domain of Vanilloid Receptor I in Channel Gating

Overview

Journal J Neurosci

Specialty Neurology

Date 2007 Oct 26

PMID 17959807

Citations 43

Authors

Nuria Garcia-Sanz

Pierluigi Valente

Ana Gomis

Asia Fernandez-Carvajal

Gregorio Fernandez-Ballester

Felix Viana

Carlos Belmonte

Antonio Ferrer-Montiel

Affiliations

Soon will be listed here.

Abstract

Transient receptor potential vanilloid receptor subtype 1 (TRPV1) is an ionotropic receptor activated by temperature and chemical stimuli. The C-terminal region that is adjacent to the channel gate, recognized as the TRP domain, is a molecular determinant of receptor assembly. However, the role of this intracellular domain in channel function remains elusive. Here, we show that replacement of the TRP domain of TRPV1 with the cognate region of TRPV channels (TRPV2-TRPV6) did not affect receptor assembly and trafficking to the cell surface, although those receptors containing the TRP domain of the distantly related TRPV5 and TRPV6 did not display ion channel activity. Notably, functional chimeras exhibited an impaired sensitivity to the activating stimuli, consistent with a significant contribution of this protein domain to channel function. At variance with TRPV1, voltage-dependent gating of chimeric channels could not be detected in the absence of capsaicin and/or heat. Biophysical analysis of functional chimeras revealed that the TRP domain appears to act as a molecular determinant of the activation energy of channel gating. Together, these findings uncover a role of the TRP domain in intersubunit interactions near the channel gate that contribute to the coupling of stimulus sensing to channel opening.

Citing Articles

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Molecular Dynamic Simulations Reveal the Activation Mechanisms of Oxidation-Induced TRPV1.

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TRPV1 in chronic pruritus and pain: Soft modulation as a therapeutic strategy.

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A Potential Route of Capsaicin to Its Binding Site in the TRPV1 Ion Channel.

Domene C, Darre L, Oakes V, Gonzalez-Resines S J Chem Inf Model. 2022; 62(10):2481-2489.

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