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Synaptosome Proteomics

Overview
Journal Subcell Biochem
Publisher Springer
Specialty Biochemistry
Date 2007 Oct 24
PMID 17953392
Citations 49
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Abstract

Our knowledge of the complex synaptic proteome and its relationship to physiological or pathological conditions is rapidly expanding. This has been greatly accelerated by the application of various evolving proteomic techniques, enabling more efficient protein resolution, more accurate protein identification, and more comprehensive characterization of proteins undergoing quantitative and qualitative changes. More recently, the combination of the classical subcellular fractionation techniques for the isolation of synaptosomes from the brain with the various proteomic analyses has facilitated this effort. This has resulted from the enrichment of many low abundant proteins comprising the fundamental structure and molecular machinery of brain neurotransmission and neuroplasticity. The analysis of various subproteomes obtained from the synapse, such as synaptic vesicles, synaptic membranes, presynaptic particles, synaptodendrosomes, and postsynaptic densities (PSD) holds great promise for improving our understanding of the temporal and spatial processes that coordinate synaptic proteins in closely related complexes under both normal and diseased states. This chapter will summarize a selection of recent studies that have drawn upon established and emerging proteomic technologies, along with fractionation techniques that are essential to the isolation and analysis of specific synaptic components, in an effort to understand the complexity and plasticity of the synapse proteome.

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References
1.
Mouledous L, Hunt S, Harcourt R, Harry J, Williams K, Gutstein H . Proteomic analysis of immunostained, laser-capture microdissected brain samples. Electrophoresis. 2003; 24(1-2):296-302. DOI: 10.1002/elps.200390026. View

2.
Akimoto Y, Comer F, Cole R, Kudo A, Kawakami H, Hirano H . Localization of the O-GlcNAc transferase and O-GlcNAc-modified proteins in rat cerebellar cortex. Brain Res. 2003; 966(2):194-205. DOI: 10.1016/s0006-8993(02)04158-6. View

3.
Leng A, Feldon J, Ferger B . Long-term social isolation and medial prefrontal cortex: dopaminergic and cholinergic neurotransmission. Pharmacol Biochem Behav. 2004; 77(2):371-9. DOI: 10.1016/j.pbb.2003.11.011. View

4.
Husi H, Ward M, Choudhary J, Blackstock W, Grant S . Proteomic analysis of NMDA receptor-adhesion protein signaling complexes. Nat Neurosci. 2000; 3(7):661-9. DOI: 10.1038/76615. View

5.
Kim S, Voshol H, van Oostrum J, Hastings T, Cascio M, Glucksman M . Neuroproteomics: expression profiling of the brain's proteomes in health and disease. Neurochem Res. 2004; 29(6):1317-31. DOI: 10.1023/b:nere.0000023618.35579.7c. View