Platelet-activating Factor Changes in Phospholipid Extracts from the Plasma, Peripheral Blood Mononuclear Cells and Bone Marrow Mononuclear Cells of Patients with Acute Leukemia--a 31P MRS in Vitro Study

Overview

Journal Cell Mol Biol Lett

Publisher Biomed Central

Specialties Biochemistry
Cell Biology
Molecular Biology

Date 2007 Oct 24

PMID 17952375

Citations 1

Authors

Malgorzata Kuliszkiewicz-Janus

Mariusz Adam Tuz

Marek Kielbinski

Stanislaw Baczynski

Bozena Jazwiec

Helena Sladowska

Affiliations

Soon will be listed here.

Abstract

The aim of this investigation was to evaluate the changes in PAF concentrations in the plasma, PBMC and BMMC of patients with acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML). The plasma was from 23 healthy volunteers (HV) and 44 patients with AL (16 ALL, 28 AML). The PBMC were from 15 HV and 55 patients with AL (18 ALL, 37 AML), and the BMMC from 40 patients with AL (11 ALL, 29 AML). Methanol-chloroform phospholipid extraction from 60 x 10(6) cells (PBMC or BMMC) was performed according to a modified version of Folch's method. (31)P MRS data was obtained on an AMX 300 Bruker spectrometer (7.05 T). The PAF concentration in the plasma of the patients with ALL or AML was lower than that for the healthy volunteers. The PAF concentration in the plasma of the patients with ALL did not differ significantly from that of the patients with AML. In the case of both the PBMC and BMMC, the PAF concentration was significantly diminished in patients with ALL relative to the concentration for those with AML and for the healthy volunteers. No differences were observed in the PAF concentrations for the AML patients and the healthy volunteers.

Citing Articles

31P MRS analysis of the phospholipid composition of the peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) of patients with acute leukemia (AL).

Kuliszkiewicz-Janus M, Tuz M, Kielbinski M, Jazwiec B, Niedoba J, Baczynski S Cell Mol Biol Lett. 2008; 14(1):35-45.

PMID: 18839072 PMC: 6275883. DOI: 10.2478/s11658-008-0032-7.

References

Chignard M, Le Couedic J, Tence M, Vargaftig B, Benveniste J . The role of platelet-activating factor in platelet aggregation. Nature. 1979; 279(5716):799-800. DOI: 10.1038/279799a0. View

Denizot Y, Dupuis F, Trimoreau F, Praloran V, Liozon E . Decreased levels of platelet-activating factor in blood of patients with lymphoid and nonlymphoid hematologic malignancies. Blood. 1995; 85(10):2992-3. View

Berdel W, Kulimova E, Kolkmeyer A, Zuhlsdorf M, Serve H, Buchner T . Receptor for platelet-activating factor (PAF) is not detectable by flow cytometry on the surface of myeloid leukemic cells. Ann Hematol. 2005; 84(12):771-3. DOI: 10.1007/s00277-005-1088-3. View

Foa R, Bussolino F, Ferrando M, Guarini A, Tetta C, Mazzone R . Release of platelet-activating factor in human leukemia. Cancer Res. 1985; 45(9):4483-5. View

Reynaud S, Malissein E, Donnard M, Bordessoule D, Turlure P, Trimoreau F . Functional platelet-activating factor receptors in immature forms of leukemic blasts. Leuk Res. 2006; 31(3):399-402. DOI: 10.1016/j.leukres.2006.06.002. View

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

Jackson J, Bolognese B, Mangar C, Hubbard W, Marshall L, Winkler J . The role of platelet activating factor and other lipid mediators in inflammatory angiogenesis. Biochim Biophys Acta. 1998; 1392(1):145-52. DOI: 10.1016/s0005-2760(98)00012-5. View

Trimoreau F, Guglielmi L, Touati M, Faucher J, Bordessoule D, Denizot Y . Platelet-activating factor receptors on B cells of chronic lymphocytic leukaemia patients. Br J Haematol. 2001; 115(3):711-2. DOI: 10.1046/j.1365-2141.2001.03138-3.x. View

Prpic V, Uhing R, Weiel J, Jakoi L, Gawdi G, Herman B . Biochemical and functional responses stimulated by platelet-activating factor in murine peritoneal macrophages. J Cell Biol. 1988; 107(1):363-72. PMC: 2115187. DOI: 10.1083/jcb.107.1.363. View

10.

Rola-Pleszczynski M, Pouliot C, Turcotte S, Pignol B, Braquet P, Bouvrette L . Immune regulation by platelet-activating factor. I. Induction of suppressor cell activity in human monocytes and CD8+ T cells and of helper cell activity in CD4+ T cells. J Immunol. 1988; 140(10):3547-52. View

11.

McIntyre T, Zimmerman G, Prescott S . Leukotrienes C4 and D4 stimulate human endothelial cells to synthesize platelet-activating factor and bind neutrophils. Proc Natl Acad Sci U S A. 1986; 83(7):2204-8. PMC: 323260. DOI: 10.1073/pnas.83.7.2204. View

12.

Kuliszkiewicz-Janus M, Tuz M, Baczynski S, Prajs I, Jazwiec B . 31P MRS analysis of the phospholipid composition of normal human peripheral blood mononuclear cells (PBMC). Cell Mol Biol Lett. 2005; 10(3):373-82. View

13.

Denizot Y, Guglielmi L, Donnard M, Trimoreau F . Platelet-activating factor and normal or leukaemic haematopoiesis. Leuk Lymphoma. 2003; 44(5):775-82. DOI: 10.1080/1042819031000067549. View

14.

Donnard M, Guglielmi L, Turlure P, Piguet C, Couraud M, Bordessoule D . Membrane and intracellular platelet-activating factor receptor expression in leukemic blasts of patients with acute myeloid and lymphoid leukemia. Stem Cells. 2002; 20(5):394-401. DOI: 10.1634/stemcells.20-5-394. View

15.

Blank M, Snyder F, BYERS L, Brooks B, MUIRHEAD E . Antihypertensive activity of an alkyl ether analog of phosphatidylcholine. Biochem Biophys Res Commun. 1979; 90(4):1194-200. DOI: 10.1016/0006-291x(79)91163-x. View

16.

Guglielmi L, Trimoreau F, Donnard M, Jaccard A, Bordessoule D, Denizot Y . Presence of membrane platelet-activating factor receptors on B cells of chronic B cell leukaemia patients. Leuk Lymphoma. 2003; 44(6):1087-8. DOI: 10.1080/1042819031000083352. View

17.

Dupuis F, Rougier F, Trimoreau F, Ostyn E, Dulery C, Praloran V . Production and metabolism of platelet-activating factor by human bone marrow cells. Res Immunol. 1997; 148(2):119-26. DOI: 10.1016/s0923-2494(97)82483-2. View

18.

Demopoulos C, Pinckard R, HANAHAN D . Platelet-activating factor. Evidence for 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine as the active component (a new class of lipid chemical mediators). J Biol Chem. 1979; 254(19):9355-8. View

19.

Garcia M, Garcia C, Gijon M, Mollinedo F, Sanchez Crespo M . Metabolism of platelet-activating factor in human haematopoietic cell lines. Differences between myeloid and lymphoid cells. Biochem J. 1991; 273 ( Pt 3):573-8. PMC: 1149801. DOI: 10.1042/bj2730573. View

20.

Denizot Y, Trimoreau F, Dupuis F, Verger C, Praloran V . PAF and haematopoiesis: III. Presence and metabolism of platelet-activating factor in human bone marrow. Biochim Biophys Acta. 1995; 1265(1):55-60. DOI: 10.1016/0167-4889(94)00193-i. View