Phosphorylation of Arabidopsis Response Regulator 7 (ARR7) at the Putative Phospho-accepting Site is Required for ARR7 to Act As a Negative Regulator of Cytokinin Signaling

Overview

Journal Planta

Specialty Biology

Date 2007 Oct 17

PMID 17938953

Citations 23

Authors

Dong Ju Lee

Sunmi Kim

Young-min Ha

Jungmook Kim

Affiliations

Soon will be listed here.

Abstract

Cytokinins are plant hormones that regulate diverse aspects of plant growth and development. Arabidopsis cytokinin signal transduction utilizes a multi-step two-component signaling (TCS) system by histidyl-aspartidyl phosphorelays. We here show that phosphorylation of ARR7, an A-type response regulator that acts as a negative regulator of cytokinin signaling, is required for its function in plants. Phosphorylation of ARR7 is inhibited in vitro by mutation in a putative phospho-accepting Asp residue into an Asn residue (ARR7(D85N)). While ectopic expression of ARR7 decreases root-growth inhibition, callus formation, and cytokinin-inducible gene expression, overexpression of ARR7 ( D85N ) at the similar level does not generate these phenotypes. ARR7(D85N) is localized to the nucleus and the half-life of this mutant protein is similar to that of ARR7 in Arabidopsis mesophyll protoplasts. These results suggest that the phosphorylation of ARR7 is necessary for ARR7-mediated cytokinin response.

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