De Novo Replication of the Influenza Virus RNA Genome is Regulated by DNA Replicative Helicase, MCM

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2007 Oct 13

PMID 17932485

Citations 57

Authors

Atsushi Kawaguchi

Kyosuke Nagata

Affiliations

Soon will be listed here.

Abstract

By dissecting and reconstituting a cell-free influenza virus genome replication system, we have purified and identified the minichromosome maintenance (MCM) complex, which is thought to be a DNA replicative helicase, as one of the host factors that regulate the virus genome replication. MCM interacted with the PA subunit of the viral RNA-dependent RNA polymerase that is found to be involved in the replication genetically. The virus genome replication was decreased in MCM2 knockdown cells. The viral polymerase appeared to be a nonproductive complex, that is, it was capable of initiating replication but produced only abortive short RNA chains. MCM stimulated de novo-initiated replication reaction by stabilizing a replication complex during its transition from initiation to elongation. Based on the findings, including the result that the MCM-mediated RNA replication reaction was competed with exogenously added RNA, we propose that MCM functions as a scaffold between the nascent RNA chains and the viral polymerase.

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