PBmice: an Integrated Database System of PiggyBac (PB) Insertional Mutations and Their Characterizations in Mice

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2007 Oct 13

PMID 17932058

Citations 26

Authors

Ling V Sun

Ke Jin

Yiming Liu

Wenwei Yang

Xing Xie

Lin Ye

Li Wang

Lin Zhu

Sheng Ding

Yi Su

Jie Zhou

Min Han

Yuan Zhuang

Tian Xu

Xiaohui Wu

Ning Gu

Yang Zhong

Affiliations

Soon will be listed here.

Abstract

DNA transposon piggyBac (PB) is a newly established mutagen for large-scale mutagenesis in mice. We have designed and implemented an integrated database system called PBmice (PB Mutagenesis Information CEnter) for storing, retrieving and displaying the information derived from PB insertions (INSERTs) in the mouse genome. This system is centered on INSERTs with information including their genomic locations and flanking genomic sequences, the expression levels of the hit genes, and the expression patterns of the trapped genes if a trapping vector was used. It also archives mouse phenotyping data linked to INSERTs, and allows users to conduct quick and advanced searches for genotypic and phenotypic information relevant to a particular or a set of INSERT(s). Sequence-based information can be cross-referenced with other genomic databases such as Ensembl, BLAST and GBrowse tools used in PBmice offer enhanced search and display for additional information relevant to INSERTs. The total number and genomic distribution of PB INSERTs, as well as the availability of each PB insertional LINE can also be viewed with user-friendly interfaces. PBmice is freely available at http://www.idmshanghai.cn/PBmice or http://www.scbit.org/PBmice/.

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