The Listeria Monocytogenes Homolog of the Escherichia Coli Era Gene is Involved in Adhesion to Inert Surfaces

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2007 Oct 9

PMID 17921262

Citations 4

Authors

Frederic Auvray

Danielle Chassaing

Cecile Duprat

Brigitte Carpentier

Affiliations

Soon will be listed here.

Abstract

Two transposon-insertional mutants of Listeria monocytogenes showing smaller viable surface-attached cell populations after disinfection with N,N-didecyl-N,N-dimethylammonium chloride were identified. In both mutants, transposon Tn917-lac was found to be inserted into the same gene, lmo1462, which is homologous to the essential Escherichia coli era gene. Both L. monocytogenes lmo1462-disrupted mutants displayed lower growth rates, as was also shown for several E. coli era mutants, and the lmo1462 gene was able to complement the growth defect of an E. coli era mutant. We showed that the disruption of lmo1462 decreased the ability of L. monocytogenes cells to adhere to stainless steel. Our results suggest that this era-like gene is involved in adhesion and contributes to the presence of L. monocytogenes on surfaces.

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