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Identification of Twinfilin-2 As a Factor Involved in Neurite Outgrowth by RNAi-based Screen

Overview
Publisher Elsevier
Specialty Biochemistry
Date 2007 Oct 4
PMID 17910947
Citations 15
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Abstract

Using RNA interference (RNAi) to suppress gene expression, we attempted to identify tyrosine kinases involved in the extension of neurites from SH-SY5Y cells. A comprehensive analysis of gene "knock-down" profiles with small interfering RNAs (siRNAs) revealed candidate proteins that might control neurite extension. Phenotype-based screening of differentiating SH-SY5Y cells following retinoic acid (RA) stimulation indicated that twinfilin-2 is a protein that is involved in neurite outgrowth, as confirmed by morphological analysis of twinfilin-2-overexpressing cells.

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