Association of Putative Pathogenicity Genes with Adherence Characteristics and Fimbrial Genotypes in Typical Enteroaggregative Escherichia Coli from Patients with and Without Diarrhoea in the United Kingdom

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2007 Sep 28

PMID 17899229

Citations 18

Authors

C Jenkins

H Chart

G A Willshaw

T Cheasty

D S Tompkins

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to compare genotypic characteristics seen in typical EAggEC isolated during a study of intestinal infectious disease from cases and controls, and to identify which genes, or combinations of genes, were most associated with diarrhoeal disease. We also investigated the association of genotype with certain characteristics, such as presence of fimbrial genes and adherence to Hep-2 cells. The aafC gene, encoding the usher for AAFII, was the only gene significantly associated with patients with diarrhoea (P < 0.005), and the aggC gene, which encodes the usher for AAFI, was the only gene significantly associated with the healthy control group (P < 0.002). Putative virulence genes significantly associated with aggregative adherence included aafC, aggR, pet, pic and astA. The shf, pet and astA genes were all more likely to be associated with type II fimbriae than with type I. We conclude that in addition to presence and absence of certain genes, studies of EAggEC pathogenicity should investigate the combinations and associations of putative virulence factors.

Citing Articles

Antibiofilm agents with therapeutic potential against enteroaggregative Escherichia coli.

Kwasi D, Babalola C, Olubiyi O, Hoffmann J, Uzochukwu I, Okeke I PLoS Negl Trop Dis. 2022; 16(10):e0010809.

PMID: 36201560 PMC: 9578610. DOI: 10.1371/journal.pntd.0010809.

Molecular Epidemiology of Enteroaggregative (EAEC) Isolates of Hospitalized Children from Bolivia Reveal High Heterogeneity and Multidrug-Resistance.

Joffre E, Iniguez Rojas V Int J Mol Sci. 2020; 21(24).

PMID: 33334000 PMC: 7765457. DOI: 10.3390/ijms21249543.

Surface Protein Dispersin of Enteroaggregative Binds Plasminogen That Is Converted Into Active Plasmin.

Moraes C, Longo J, Silva L, Pimenta D, Carvalho E, Morone M Front Microbiol. 2020; 11:1222.

PMID: 32625178 PMC: 7315649. DOI: 10.3389/fmicb.2020.01222.

Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease.

Ellis S, Crossman L, McGrath C, Chattaway M, Holken J, Brett B Sci Rep. 2020; 10(1):7475.

PMID: 32366874 PMC: 7198487. DOI: 10.1038/s41598-020-64424-3.

Antibiotic resistance and molecular characterization of enteroaggregative isolated from patients with diarrhea in the Eastern Province of Saudi Arabia.

Elhadi N, AlJindan R, Alsamman K, Alomar A, Aljeldah M Heliyon. 2020; 6(4):e03721.

PMID: 32274438 PMC: 7132075. DOI: 10.1016/j.heliyon.2020.e03721.

References

Zamboni A, Fabbricotti S, Fagundes-Neto U, Scaletsky I . Enteroaggregative Escherichia coli virulence factors are found to be associated with infantile diarrhea in Brazil. J Clin Microbiol. 2004; 42(3):1058-63. PMC: 356835. DOI: 10.1128/JCM.42.3.1058-1063.2004. View

Nataro J, Kaper J, Robins-Browne R, Prado V, Vial P, Levine M . Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis J. 1987; 6(9):829-31. DOI: 10.1097/00006454-198709000-00008. View

Cobeljic M, Miljkovic-Selimovic B, Velickovic Z, Lepsanovic Z, Zec N, Savic D . Enteroaggregative Escherichia coli associated with an outbreak of diarrhoea in a neonatal nursery ward. Epidemiol Infect. 1996; 117(1):11-6. PMC: 2271665. DOI: 10.1017/s0950268800001072. View

Sethi D, Wheeler J, Cowden J, Rodrigues L, Sockett P, Roberts J . A study of infectious intestinal disease in England: plan and methods of data collection. Commun Dis Public Health. 1999; 2(2):101-7. View

Eslava C, Navarro-Garcia F, Czeczulin J, Henderson I, Cravioto A, Nataro J . Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli. Infect Immun. 1998; 66(7):3155-63. PMC: 108327. DOI: 10.1128/IAI.66.7.3155-3163.1998. View

Elias W, Barros S, Moreira C, Trabulsi L, Gomes T . Enteroaggregative Escherichia coli strains among classical enteropathogenic Escherichia coli O serogroups. J Clin Microbiol. 2002; 40(9):3540-1. PMC: 130765. DOI: 10.1128/JCM.40.9.3540-3541.2002. View

Schmidt H, Knop C, Franke S, Aleksic S, Heesemann J, Karch H . Development of PCR for screening of enteroaggregative Escherichia coli. J Clin Microbiol. 1995; 33(3):701-5. PMC: 228017. DOI: 10.1128/jcm.33.3.701-705.1995. View

Schubert S, Rakin A, Karch H, Carniel E, Heesemann J . Prevalence of the "high-pathogenicity island" of Yersinia species among Escherichia coli strains that are pathogenic to humans. Infect Immun. 1998; 66(2):480-5. PMC: 107931. DOI: 10.1128/IAI.66.2.480-485.1998. View

Nataro J, Kaper J . Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1):142-201. PMC: 121379. DOI: 10.1128/CMR.11.1.142. View

10.

Heilmann C, Schweitzer O, Gerke C, Vanittanakom N, Mack D, Gotz F . Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol. 1996; 20(5):1083-91. DOI: 10.1111/j.1365-2958.1996.tb02548.x. View

11.

Paiva de Sousa C, Dubreuil J . Distribution and expression of the astA gene (EAST1 toxin) in Escherichia coli and Salmonella. Int J Med Microbiol. 2001; 291(1):15-20. DOI: 10.1078/1438-4221-00097. View

12.

Nishi J, Sheikh J, Mizuguchi K, Luisi B, Burland V, Boutin A . The export of coat protein from enteroaggregative Escherichia coli by a specific ATP-binding cassette transporter system. J Biol Chem. 2003; 278(46):45680-9. DOI: 10.1074/jbc.M306413200. View

13.

Bellini E, Elias W, Gomes T, Tanaka T, Taddei C, Huerta R . Antibody response against plasmid-encoded toxin (Pet) and the protein involved in intestinal colonization (Pic) in children with diarrhea produced by enteroaggregative Escherichia coli. FEMS Immunol Med Microbiol. 2005; 43(2):259-64. DOI: 10.1016/j.femsim.2004.08.008. View

14.

Mathewson J, Johnson P, DuPont H, Satterwhite T, Winsor D . Pathogenicity of enteroadherent Escherichia coli in adult volunteers. J Infect Dis. 1986; 154(3):524-7. DOI: 10.1093/infdis/154.3.524. View

15.

Piva I, Pereira A, Ferraz L, Silva R, Vieira A, Blanco J . Virulence markers of enteroaggregative Escherichia coli isolated from children and adults with diarrhea in Brasília, Brazil. J Clin Microbiol. 2003; 41(5):1827-32. PMC: 154701. DOI: 10.1128/JCM.41.5.1827-1832.2003. View

16.

Law D, Chart H . Enteroaggregative Escherichia coli. J Appl Microbiol. 1998; 84(5):685-97. DOI: 10.1046/j.1365-2672.1998.00372.x. View

17.

Nishikawa Y, Ogasawara J, Helander A, Haruki K . An outbreak of gastroenteritis in Japan due to Escherichia coli O166. Emerg Infect Dis. 1999; 5(2):300. PMC: 2640687. DOI: 10.3201/eid0502.990220. View

18.

Itoh Y, Nagano I, Kunishima M, Ezaki T . Laboratory investigation of enteroaggregative Escherichia coli O untypeable:H10 associated with a massive outbreak of gastrointestinal illness. J Clin Microbiol. 1997; 35(10):2546-50. PMC: 230008. DOI: 10.1128/jcm.35.10.2546-2550.1997. View

19.

Sheikh J, Czeczulin J, Harrington S, Hicks S, Henderson I, Le Bouguenec C . A novel dispersin protein in enteroaggregative Escherichia coli. J Clin Invest. 2002; 110(9):1329-37. PMC: 151617. DOI: 10.1172/JCI16172. View

20.

Pereira A, Ferraz L, Silva R, Giugliano L . Enteroaggregative Escherichia coli virulence markers: positive association with distinct clinical characteristics and segregation into 3 enteropathogenic E. coli serogroups. J Infect Dis. 2007; 195(3):366-74. DOI: 10.1086/510538. View